| 断喙应激对雏鸡胸腺细胞凋亡及相关凋亡蛋白表达的影响 |
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| 引用本文： | 孙桂荣,李燕,康相涛,田亚东,张虎,李奎.断喙应激对雏鸡胸腺细胞凋亡及相关凋亡蛋白表达的影响[J].畜牧兽医学报,2011,42(7). |
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| 作者姓名： | 孙桂荣 李燕 康相涛 田亚东 张虎 李奎 |
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| 作者单位： | 河南农业大学牧医工程学院,郑州,450002 |
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| 基金项目： | 国家农业科技成果转化项目(2009GB2D000218); 现代农业产业技术体系建设专项资金(nycytx-41-g05) |
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| 摘 要： | 为研究断喙应激对雏鸡胸腺细胞凋亡及相关凋亡蛋白表达的影响,采用电子显微镜技术、流式细胞术和免疫组织化学技术分析断喙对鸡胸腺淋巴细胞的细胞增殖、分化、凋亡及相关凋亡蛋白(Bcl-2和Bax)表达的影响.结果表明:采用电子显微镜技术观察到断喙对胸腺淋巴细胞产生明显的影响,断喙组胸腺细胞内可看到细胞核明显的应激反应,细胞核凝集,较致密,部分细胞核即将溶解,并出现一定数量的凋亡和坏死细胞；流式细胞仪检测表明对照组和断喙组的胸腺内淋巴细胞都是以G1期为主,但断喙组G1期淋巴细胞数量比试验组高；对照组和断喙组胸腺淋巴细胞凋亡率在断喙后5d时差异显著(P＜0.05)；免疫组化结果表明断喙应激没有影响Bcl-2和Bax蛋白在免疫器官内的表达部位；但有降低Bcl 2在胸腺细胞内表达量的趋势；有提高Bax在胸腺细胞内表达量的趋势.结果表明断喙应激促进了雏鸡胸腺淋巴细胞凋亡.
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| 关 键 词： | 雏鸡 断喙应激 胸腺 细胞凋亡 凋亡蛋白 |
Effect of Beak Trimming on the Apoptosis and Related Protein Expression of Chicken Thymus |
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| SUN Gui-rong
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LI Yan
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KANG Xiang-tao
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TIAN Ya-dong
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ZHANG Hu
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LI Kui.Effect of Beak Trimming on the Apoptosis and Related Protein Expression of Chicken Thymus[J].Acta Veterinaria et Zootechnica Sinica,2011,42(7). |
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| Authors: | SUN Gui-rong LI Yan KANG Xiang-tao TIAN Ya-dong ZHANG Hu LI Kui |
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| Institution: | SUN Gui-rong,LI Yan,KANG Xiang-tao,TIAN Ya-dong,ZHANG Hu,LI Kui (College of Livestock Husbandry and Veterinary Engineering,Henan Agricultural University,Henan Research Center of Breeding Resourcesfor Poultry,Zhengzhou 450002,China) |
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| Abstract: | In order to identify the effect of beak trimming on the apoptosis and related proteins expression of young chicken thymus,the cell proliferation,cell differentiation,cell apoptosis and the expression of apoptosis-related protein(Bcl-2 and Bax) in chicken thymus were studied by using the electron microscope,flow cytometry,immunohistochemical technique.The results of electronic microscope showed that significant stress response could be found in chicken thymus cells after beak trimming.Cell nuclear agglutinat... |
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| Keywords: | chicken beak trimming thymus cell apoptosis apoptosis protein |
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