HIF-1α和IL-17在牦牛、犏牛及黄牛睾丸中的表达比较研究

本研究旨在比较HIF-1α和IL-17在不同牛睾丸组织中的差异性表达，进一步探究HIF-1α与IL-17在牛睾丸组织中发挥调控功能的关联性。以成年牦牛、犏牛和黄牛的睾丸作为研究对象，采用Western-blot、实时荧光定量PCR法检测HIF-1α及IL-17蛋白和基因在3种牛睾丸组织的表达差异。结果显示，3种牛睾丸组织中HIF-1α与IL-17基因和蛋白表达都存在显著性差异，且在犏牛睾丸组织中的表达均极显著高于牦牛和黄牛（P<0.01）；此外，牦牛HIF-1α与IL-17蛋白水平表达显著高于黄牛（P<0.05，P<0.01），而牦牛IL-17基因水平表达与黄牛差异不显著（P>0.05）。HIF-1α和IL-17基因和蛋白在不同牛睾丸组织中的表达差异说明其具有种属特异性；而牦牛和犏牛睾丸组织中显著性高表达提示HIF-1α和IL-17对适应低氧环境具有重要的调节作用。

Comparative Study on the Expression of HIF-1α and IL-17 in Testes of Yak,Cattle-Yak and Yellow Cattle

The purpose of this study was to compare the differential expression of HIF-1α and IL-17 in the testicular tissues of different bovines, and to further explore the correlation between the regulatory functions of HIF-1α and IL-17 in bovine testes. The testes of adult yak, cattle-yak,and yellow cattle were used as the research objects. Western-blot and real-time quantitative PCR were used to detect the differential expression of HIF-1α and IL-17 proteins and genes in 3 types of bovine testes, respectively. The results showed that the expression of HIF-1α and IL-17 genes and proteins were significantly different among 3 bovine testicular tissues, and the expression in cattle-yak testes were extremely significantly higher than those in yak and yellow cattle (P<0.01); In addition, the expression levels of HIF-1α and IL-17 proteins in yak were significantly higher than those of yellow cattle (P<0.05,P<0.01), while the expression level of IL-17 gene was not significantly different between yak and yellow cattle (P>0.05). The differences in expression of HIF-1α and IL-17 genes and proteins in different bovine testicular tissues indicated species specificity; The significantly higher expression of HIF-1α and IL-17 in testis tissues of yak and cattle-yak suggested that they might have important regulatory effects on the adaptation to hypoxic environment.