维生素E对秦川牛冷冻精液的抗氧化保护作用

本试验旨在研究维生素E对秦川牛细管冷冻精液品质和精浆中抗氧化酶活性的影响.在稀释液中分别添加0.00、0.02、0.04、0.06、0.08、0.10 mg·mL~(-1)的维生素E,将细管冻精解冻(37.5℃,30 s)后用伟力精子分析系统分析精子活力和精子顶体完整率等标准参数,并用相关试剂盒测定精浆中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH)、谷胱甘肽还原酶(GR)和过氧化氢酶(CAT)的活性.结果表明:当维生素E添加量为0.04mg·mL~(-1)时,精子活力与对照组相比差异显著(P<0.05),SOD、GSH、CAT 3种酶活性与对照组相比差异显著(P<0.05).当维生素E的添加量为0.06 mg·mL~(-1)时,冷冻-解冻后精子活力显著高于对照组(P<0.05),顶体完整率极显著高于对照组(P<0.01);GR和CAT酶活性与对照组相比均差异显著(P<0.05).当维生素E的添加量为0.08 mg·mL~(-1)时,精子活力、活率及顶体完整率与对照组相比差异显著(P<0.05),GSH与对照相组比差异极显著(P<0.01),GR及CAT与对照组相比差异显著(P<0.05).在冷冻精液稀释液中添加维生素E可以有效提高冷冻-解冻后秦川牛精液品质及精浆中SOD、CAT、GSH、GR酶的活性,稀释液中维生素E的适宜添加量为0.04～0.08 mg·mL~(-1).

The Antioxidation Protective Effects of Vitamin E on Frozen-thawed Semen in Qinchuan Cattle

The aims of this study were to determine the antioxidation protective effects of vitamin E on the quality of straw frozen semen and antioxidant enzyme activity in seminal plasma of Qinchuan cattle. In this experiment, the vitamin E with different concentrations (0.00, 0.02, 0.04, 0.06, 0.08, 0. 10 mg·mL~(-1) were added in the ordinary dilution. After thawing the straw frozen semen(37.5℃,30 s), the motility of sperm, the integrity rate of sperm acrosome and other parameters were analyzed by WEILI sperm analysalor. Then the activity of SOD, GSH, GR and CAT were detected by the related kit. The results showed that when the addition of vitamin E was 0.04 mg·mL~(-1), there were significant differences between experiment and control groups in motility of sperm (P<0.05) and also had significant differences in enzyme activity of SOD, GSH and CAT (P<0.05). When the addition of vitamin E was 0.06 mg·mL~(-1), the motility of sperm were significant higher than that of control group, the integrity rate of sperm acrosome was significant higher than that of control group (P<0.01); there were significant differences between experiment and control groups in the activity of GR and CAT. When the vitamin E concentration was 0.08 mg·mL~(-1), the sperm motility, the motility and the integrity rate of acrosome had very significant differences (P<0.01) between experiment and control groups, there were significant differences in GR and CAT activity between experiment and control groups. With the addition of vitamin E in the dilution of frozen semen, the quality of frozen-thawed semen and enzyme activity of SOD, CAT, GSH and GR in seminal plasma of Qinchuan cattle can be effectively improved, and the appropriate additions of vitamin E in ordinary dilution were 0. 04-0. 08 mg·mL~(-1).