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| RNAi表达载体对avUCP基因表达的抑制作用 | |
| 引用本文： | 仲明,张宏福,董枫岚,王润平,刘燕,杨琳.RNAi表达载体对avUCP基因表达的抑制作用[J].畜牧兽医学报,2007,38(4):352-355. |
| 作者姓名： | 仲明 张宏福 董枫岚 王润平 刘燕 杨琳 |
| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京,100094;华南农业大学动物科技学院,广州,510642 2. 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京,100094 3. 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京,100094;中国农业大学生物学院,北京,100094 4. 华南农业大学动物科技学院,广州,510642 |
| 基金项目： | “863”计划资助项目（2003AA243046）;“973”计划资助项目（2004CB117507）.致谢：试验过程中得到了中山大学生命科学学院有害生物控制与资源利用国家重点实验室和张文庆教授的支持与帮助,在此表达深深的谢意和感激. |
| 摘 要： | 研究RNA干扰（RNA interference,RNAi）表达载体对UCP基因的抑制作用。针对鸡解耦联蛋白（avianuncoupling protein,av UCP）基因,构建4种RNAi质粒表达载体（分别称为A、B、C和D号载体）。再经过脂质体法转染成肌纤维细胞,应用荧光定量PCR及流式细胞术检测其对av UCP mRNA及蛋白表达的影响。构建的4种表达载体均抑制了av UCP的mRNA及蛋白的表达,其中转染C号载体的成肌纤维细胞av UCPmRNA表达相当于空白对照组的14.6%,蛋白抑制率达93.7%。RNAi表达载体可以有效的抑制avUCP基因的表达。 |
| 关 键 词： | RNA干扰 avUCP 表达载体 FQ-PCR 成肌纤维 |
| 文章编号： | 0366-6964（2007）04-0352-04 |
| 修稿时间： | 2006-03-20 |
The Inhibitory Effects of siRNA Expression Vector on the Expression of avUCP Gene |
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| ZHONG Ming,ZHANG Hong-fu,DONG Feng-lan,WANG Run-ping,LIU Yan,YANG Lin.The Inhibitory Effects of siRNA Expression Vector on the Expression of avUCP Gene[J].Acta Veterinaria et Zootechnica Sinica,2007,38(4):352-355. | |
| Authors: | ZHONG Ming ZHANG Hong-fu DONG Feng-lan WANG Run-ping LIU Yan YANG Lin |
| Institution: | 1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciencl, Chinese Academy of Agricultunal Sciences ,Beijing 100094, China; 2. College of Animal Science and Technology, Southern China Agricultural University, Guangzhou 510642, China; 3. College of Biology, China Agricultural University, Beijing 100094, China |
| Abstract: | To investigate the inhibitory effects of RNAi(RNA interference) expression vector on the expression of avUCP gene.Four siRNA expression vectors(A,B,C and D) were constructed to be aimed directly at avUCP gene.The recombinants were transfected into myofibroblast with liposomes.Expression of avUCP was detected with fluorescence quantitative PCR(FQ-PCR)and flow cytometry.Four kinds of expression vectors could reduce the expression of avUCP mRNA and protein all in myofibroblast.The expression of avUCP mRNA reduced to 14.6% of control group,and the protein inhibition ratio reached 93.7%.The RNAi expression vector can effectively inhibit the expression of avUCP gene. |
| Keywords: | RNA interference(RNAi) avUCP expression vector fluorescence quantitative PCR(FQ-PCR) myofibroblast |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |