| 正义和反义Calpastatin cDNA真核表达载体的构建 |
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| 引用本文： | 杜敏,南庆贤,朱美君,张曼夫.正义和反义Calpastatin cDNA真核表达载体的构建[J].畜牧兽医学报,2000,31(2):97-101. |
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| 作者姓名： | 杜敏 南庆贤 朱美君 张曼夫 |
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| 作者单位： | 1. 中国农业大学食品学院,北京,100094
2. 中国农业大学生物学院,北京,100094 |
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| 基金项目： | 国家自然科学基金!(编号 396 70 5 48)，International Foundation for Science(瑞典)资助 |
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| 摘 要： | 本研究利用真核表达载体ｐＲＣ／ＲＳＶ，将位于ｐＢＳ－ＳＫ质粒中的ＣａｌｐａｓｔａｔｉｎｃＤＮＡ以正反两个方向构入载体；经酶切分析，构建的质粒结构正确。所构建质粒ｐＲＣ／ＲＳＶ正义、反义ＣａｌｐａｓｔａｔｉｎｃＤＮＡ两个表达载体，为进一步转化肌细胞，人为控制Ｃａｌｐａｓｔａｔｉｎ基因的表达，从而控制Ｃａｌｐａｉｎ活性，深入研究钙蛋白酶在肌肉发育及肉嫩化中的作用打下基础。
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| 关 键 词： | 钙蛋白酶 真核表达载体 肌肉发育 基因表达 家畜 |
THE CONSTRUCTION OF pRC/RSV SENSE AND ANTISENSE CALPASTATIN cDNA EXPRESSION VECTORS |
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| Du Min,Nan Qingxian,Zhu Meijun,Zhang Manfu.THE CONSTRUCTION OF pRC/RSV SENSE AND ANTISENSE CALPASTATIN cDNA EXPRESSION VECTORS[J].Acta Veterinaria et Zootechnica Sinica,2000,31(2):97-101. |
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| Authors: | Du Min Nan Qingxian Zhu Meijun Zhang Manfu |
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| Abstract: | WT5BZ] Calpain system has been suggested to play a key role in myoblast fusion and muscle growth.Possibly,we can enhance the growth of muscle through controlling the activity of calpains.Calpastatin is the endogenous inhibitor of calpains which regulate calpain activity in vivo .This research employs eukaryotic expression vector pRC/RSV and Calpastatin cDNA in pBS SK to construct two expression vectors in which calpastatin cDNA are inserted in sense and antisense direction respectively.Endonuclease restriction analysis proved that the two plasmids were correctly constructed.Those plasmids will be useful in regulating the activities of calpains by controlling the expression of calpastatin gene,and facilitate researches in the role of calpain system in muscle growth and meat tenderization. WT5HZ] |
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| Keywords: | Calpain Calpastatin Eukaryotic expression vector |
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