| GnRH促鸡卵泡颗粒细胞孕酮合成功能作用机制的研究 |
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| 引用本文： | 汪琳仙,李莹辉,王海滨.GnRH促鸡卵泡颗粒细胞孕酮合成功能作用机制的研究[J].畜牧兽医学报,1999,30(3):200-205. |
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| 作者姓名： | 汪琳仙 李莹辉 王海滨 |
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| 作者单位： | 中国农业大学生物学院，航天医学工程研究所 |
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| 摘 要： | 本实验选用掌握产蛋规律的莱航鸡，在排卵前１６－１８ｈ剖腹取卵泡，分离和纯化颗粒细胞。采用无血清贴壁培养法，初步探讨了鸡促性腺激素释放激素促颗粒细胞孕酮合成的作用机制。获得以下结果：（１）ＰＫＣ阻断剂Ｈ７（２５－５０μｍｏｌ／Ｌ）能阻断ＧｎＲＨ－Ⅱ促颗粒细胞Ｐ４合成作用；（２）磷酸二酯酶抑制剂ＭＩＸ（１０＾－５－１０＾－３ｍｏｌ／Ｌ）能促进Ｐ４分泌，但对ＧｎＲＨ－Ⅱ促Ｐ４合成效应无明显影响；（３）钙
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| 关 键 词： | GnRH 孕酮合成 作用机制 鸡 卵泡颗粒细胞 |
MECHANISMOFGONADOTROPINRELEASINGHORMONESTIMULATINGPROGESTERONEPRODUCTIONINCULTUREDGRANULOSACELLSOFCHICKENFOLLICLES |
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| Author WangLinxian,LiYinghui,WangHaibin.MECHANISMOFGONADOTROPINRELEASINGHORMONESTIMULATINGPROGESTERONEPRODUCTIONINCULTUREDGRANULOSACELLSOFCHICKENFOLLICLES[J].Acta Veterinaria et Zootechnica Sinica,1999,30(3):200-205. |
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| Authors: | Author WangLinxian LiYinghui WangHaibin |
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| Abstract: | ThepresentexperimentswereconductedtostudythemechanismofGnRHstimulatingprogesterone(P4)productionbyculturedchickengranulosacells(GC)Thedifferentsizeatfollicles(F1F4)wereobtainedfromlayinghens(Leghornstrain)whichwerekilledat1618hbeforeovulationTheGCswereisolatedfromthegranulosalayerswith01%collagenasefor5to7minutesandculturedinaserumfreemediumTheresultsshowthat(1)H7,(2550mol/L)couldblockGnRHinducedP4production;(2)MIX(10-510-3mol/L)alonestimulatedtheprogesteroneproduction,buthadnosignificanteffectonP4productioninducedbyGnRH;(3)P4productioninducedbyGnRHweredecreasedsignificantlyinthepresenceofEGTA(13mmol/L),butitseffectwasconvertedbyaddingCa2 15mol/L;(4)TheactionofVerapamil(thecalciumchannelblocker)suppressedP4productionstimulatedbyGnRHwasnotsignificantTheresultsmentionedaboveindicatethatthePKCsystemmaymediatetherelationofP4productionbyGnRHinchickenGC;andtheactionofintracellularcalciumisalsoimportant |
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| Keywords: | GnRH Progesterone Mechanism Granulosacells Chickenfollicles |
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