荷斯坦公牛X和Y精子核形态差异及其影响因素分析

旨在分析荷斯坦公牛X和Y精子核形态(包括精子大小和形状)及其差异,探究流式分选及分选后冷冻对公牛X和Y精子核形态的影响.本研究使用荧光显微镜拍摄15头年龄相当、健康状况良好的荷斯坦公牛X和Y精子冷冻前后及新鲜精液分选前后共6类精子核图像,利用Image J插件Nuclear Morphology Analysis分析3...

Morphological Differences of X and Y Sperm Nuclei and Their Influencing Factors in Holstein Bulls

This study was conducted to analyze the differences of nuclear morphology (including sperm size and shape) between Holstein bull X and Y sperm, clarifying the effects of freezing and flow sorting on the nuclear morphology of bull X and Y sperm. Six types of sperm nuclei images from 15 healthy Holstein bulls with the same age were captured by fluorescence microscopy for fresh and frozen non-sexed sperm, and fresh and frozen X and Y sperm. The Image J plug-in Nuclear Morphology Analysis was used to analyze the 8 indices information related to the morphology of 37 000 sperm nuclei images. The results showed that the proportion of bulls with significant difference in sperm nucleus morphology between X and Y (P<0.05) was less than 50%. After flow sorting, the sperm nucleus area, perimeter and length of 8 (8/11) bulls, the width of 6 (6/11) bulls changed significantly|The ellipticity, elongation and regularity of sperm nuclei in 5 (5/11) bulls and the circularity of sperm nuclei in 3 (3/11) bulls changed significantly. After freezing, there were significant differences in sperm nuclear area and perimeter among 11 (11/14) bulls, the length of sperm nuclei among 10 (10/14) bulls, width of sperm nuclei among 9 (9/14) bulls changed significantly|And there were significant differences in sperm nucleus circularity among 5 (5/14) bulls, regularity, elongation and ellipticity of sperm nuclei among 6 (6/14) bulls changed significantly. Only 4 bulls had significant differences in the size of X and Y sperm nuclei before freezing, but added to 8 bulls after freezing. Based on the analysis of morphological information from a large number of sperm nuclear images, it could be clarified that flow sorting and freezing had the significant impact on the nuclear size of Holstein bull sperm, and the impact of freezing on the nuclear size of Holstein bull Y sperm was higher than that of X sperm. The research will provide a reference for the improvement of sex-controlled sperm sorting and sperm freezing, and are of great significance to the improvement of bull production performance and cow economic benefit.