| 绵羊Txl-2基因CDS区克隆及其生物信息学分析 |
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| 引用本文： | 秦雪,张晓东,张国林,郭丽娜,张春香,任有蛇,岳文斌.绵羊Txl-2基因CDS区克隆及其生物信息学分析[J].黑龙江动物繁殖,2014(2):3-7. |
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| 作者姓名： | 秦雪 张晓东 张国林 郭丽娜 张春香 任有蛇 岳文斌 |
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| 作者单位： | 山西农业大学动物科技学院,山西太谷030801 |
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| 基金项目： | 现代农业产业技术体系建设专项资金（NYCYTX-39）资助. |
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| 摘 要： | 本研究以绵羊睾丸组织为材料,利用RT-PCR技术获得绵羊硫氧还蛋白类蛋白2(Thioredoxin like protein 2,Txl-2)基因的CDS区序列,并对其进行生物信息学分析。结果表明,克隆得到的绵羊Txl-2基因CDS区为795 bp,编码264个氨基酸。生物信息学分析结果表明,Txl-2编码的蛋白无跨膜区、信号肽和N-糖基化位点,存在多个磷酸化位点;预测出了Txl-2蛋白的二级结构和三级结构;Clustel W方法对比绵羊Txl-2与绵羊、山羊预测序列同源性最高。
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| 关 键 词： | Txl－2 绵羊 CDS区克隆 生物信息学分析 |
Clonging and Bioinformatics Prediction of Thioredoxin like protein 2 Coding Sequence in Sheep |
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| QIN Xue,ZHANG Xiao-dong,ZHANG Gao-lin,GUO Li-na,ZHANG Chun-xiang,REN You-she,YUE Wen-bin.Clonging and Bioinformatics Prediction of Thioredoxin like protein 2 Coding Sequence in Sheep[J].Heilongjiang Journal of Animal Reproduction,2014(2):3-7. |
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| Authors: | QIN Xue ZHANG Xiao-dong ZHANG Gao-lin GUO Li-na ZHANG Chun-xiang REN You-she YUE Wen-bin |
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| Institution: | (College of Animal Science and Technology Shanxi Agricuhral University, TaiGu 030801 ) |
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| Abstract: | RT - PCR was used to clone full length of Thioredoxin like protein 2 CDS from sheep testis and bioinformatics analysis was performed. The results showed that Txl -2 CDS was 795 bp and encoded a protein of 264 amino acids. It had no transmembrane region, signal peptide and N -glycosylation sites of Txl- 2 protein, while there were several phosphpryiation sites in the sequence. The Secondary structure and tertiary structure of Txl -2 protein were also predicted. The Clustel W alignment results showed that Txl- 2 had a close relationship with sheep and goat predicated sequence. |
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| Keywords: | Thioredoxin like protein 2 sheep CDS cloning bioinformatics analysis |
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