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| 不同冷冻方法对牛卵母细胞及孤雌胚发育的影响 | |
| 引用本文： | 卞江涛,倪明,胡高博,赵鑫,吕自力,卢全晟,薛利,宋彦军,张居农.不同冷冻方法对牛卵母细胞及孤雌胚发育的影响[J].中国草食动物,2010,30(1). |
| 作者姓名： | 卞江涛 倪明 胡高博 赵鑫 吕自力 卢全晟 薛利 宋彦军 张居农 |
| 作者单位： | 1. 石河子大学动物科技学院,新疆,832000;新疆金牛生物科学院,乌鲁木齐,830026 2. 石河子大学动物科技学院,新疆,832000 3. 石河子大学动物科技学院,新疆,832000;北京合众牧丰科技有限公司 4. 新疆金牛生物科学院,乌鲁木齐,830026 5. 北京合众牧丰科技有限公司 |
| 摘 要： | 研究了麦管和OPS管法冷冻以及OPS法中保护剂种类对牛卵母细胞体外成熟及孤雌胚发育的影响。结果发现,OPS管冷冻牛卵母细胞形态正常率、卵裂率、囊胚率极显著高于麦管法(P<0.05)。在OPS法中,应用两种保护剂冷冻后,卵母细胞形态正常率、卵裂率、囊胚率差异极显著(P<0.01);采取38℃与25℃温度平衡,冷冻后卵母细胞各发育指标差异不显著(P>0.05);采用4℃平衡,冷冻后的卵母细胞激活后没有出现囊胚,各发育指标极显著降低(P<0.01)。结果表明,OPS法可以有效地保护卵母细胞,保证其后孤雌激活;采用低温平衡会对孤雌发育的囊胚阶段有较大影响。 |
| 关 键 词： | 冷冻方法 牛卵母细胞 体外成熟 孤雌胚发育 |
Effects of different cryopreservation methods on the developmental capacity and parthenogenetic embryo proliferation of bovine follicular |
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| Bian Jiang-tao,Ni Ming,Hu Gao-bo,Zhao Xin,Lv Zi-xin,Lu Quan-sheng,Xue Li,Song Yan-jun,Zhang Ju-nong.Effects of different cryopreservation methods on the developmental capacity and parthenogenetic embryo proliferation of bovine follicular[J].China Herbivores,2010,30(1). | |
| Authors: | Bian Jiang-tao Ni Ming Hu Gao-bo Zhao Xin Lv Zi-xin Lu Quan-sheng Xue Li Song Yan-jun Zhang Ju-nong |
| Institution: | 1.College of Animal Science and Technology;Shihezi University;Xinjiang 832003;China;2.Xinjiang Jinniu Academy of Biology;Urumqi 830000;et al |
| Abstract: | The effects of different cryopreservation methods and cryoprotectants on the developmental capacity and parthenogenetic embryo proliferation of bovine follicular were compared.The results indicated that there was significantly different effects(P<0.01) on the rate of morphologically normal oocytes,cleavage rate,blastocyst rate between the open pulled straw(OPS) vitrification and the conventional-straw vitrification.After using cryoprotectants in OPS vitrification,the rate of morphologically normal oocytes,c... |
| Keywords: | cryopreservation methods bovine follicular in vitro maturation parthenogenetic embryo proliferation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |