| 牦牛PRDM9基因的CDS序列及其生物信息学分析 |
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| 引用本文： | 李铎,钟金城.牦牛PRDM9基因的CDS序列及其生物信息学分析[J].中国草食动物,2011,31(2):15-18. |
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| 作者姓名： | 李铎 钟金城 |
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| 作者单位： | 动物遗传育种学国家民委-教育部重点实验室,西南民族大学,四川成都,610041 |
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| 摘 要： | 文章通过RT-PCR技术克隆了牦牛PRDM9基因的cDNA序列,利用DNAMAN、MAGA4、ExPASy等多个生物信息学软件进行了分析研究。结果表明:扩增出的牦牛PRDM9基因的编码区长1 533 bp,编码510个氨基酸。与普通牛、人和鼠相应基因核苷酸序列进行比对,其一致性分别为99%、76%和69%。预测的牦牛PRDM9蛋白二级和三级结构显示它是一个具有3种功能结构域的弱碱性螺旋状蛋白。这为今后的进一步研究提供了理论基础。
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| 关 键 词： | 牦牛 PRDM9基因 生物信息学分析 |
CDS Cloning of PRDM9 Gene and Its Bioinformatics Analysis in Yak |
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| Li Duo,Zhong Jin-cheng.CDS Cloning of PRDM9 Gene and Its Bioinformatics Analysis in Yak[J].China Herbivores,2011,31(2):15-18. |
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| Authors: | Li Duo Zhong Jin-cheng |
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| Institution: | (Key Laboratory of Animal Genetics and Breeding,State Ethnic Affairs Commission and Ministry of Education,Southwest University for Nationalities,Chengdu 610041) |
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| Abstract: | The objectives of this study were to clone the coding region of PRDM9 gene in order to provide a theoretical basis for the study of fertilization biology in the yak by PT-PCR.The nucleotide and amino acid sequences were analyzed using DNAMAN,MAGA4 and ExPASy software.Results showed that the coding region of PRDM9 gene in yak was 1 533 bp long and encoded 510 amino acids.Compared with Bos taurus,human and mouse,the similarities of PRDM9 gene sequence in yak were 99%,76%,69%,respectirely.The coding region of PRDM9 gene in yak was successfully cloned,which provides a theoretical basis for further study on its structure and function. |
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| Keywords: | yak PRDM9 gene bioinformatics analysis |
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