| 六个家驴品种mtDNA D—Loop部分序列的遗传多样性分析 |
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| 引用本文： | 赵朝霞,刘文忠,朱文进,武建亮,吴亚权.六个家驴品种mtDNA D—Loop部分序列的遗传多样性分析[J].中国草食动物,2008,28(4). |
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| 作者姓名： | 赵朝霞 刘文忠 朱文进 武建亮 吴亚权 |
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| 作者单位： | 1. 山西农业大学,太谷,030801
2. 河北科技师范学院动物科学系 |
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| 基金项目： | 教育部"新世纪优秀人才支持计划"资助 |
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| 摘 要： | 对中国的6个家驴品种(关中驴、德州驴、庆阳驴、泌阳驴、广灵驴、晋南驴)利用PCR-SSCP技术分析了其mtDNA D-Loop部分序列的遗传多样性。87个个体共检测到19个单倍型,单倍型比例为21.8%。单倍型多样度以关中驴、晋南驴较高,分别为0.834和0.904;其次为泌阳驴和广灵驴,分别为0.629、0.529;德州驴和庆阳驴较低,分别为0.467、0.599。文章从分子水平上揭示了家驴的遗传多样性,为我国家驴品种资源的评价和利用提供了一些依据。
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| 关 键 词： | 家驴 PCR-SSCP mtDNA D-Loop 遗传多样性 |
Analysis on the Genetic Diversity of Partial Sequences in Mitochondriai DNA D-loop of Six Donkey Breeds |
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| Zhao Zhao-xia,Liu Wen-zhong,Zhu Wen-jin,et al.Analysis on the Genetic Diversity of Partial Sequences in Mitochondriai DNA D-loop of Six Donkey Breeds[J].China Herbivores,2008,28(4). |
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| Authors: | Zhao Zhao-xia Liu Wen-zhong Zhu Wen-jin |
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| Institution: | Zhao Zhao-xia1,Liu Wen-zhong1,Zhu Wen-jin2,et al |
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| Abstract: | The genetic diversity of the mitochondrial DNA(mtDNA) D-loop partial sequences from six donkey breeds(Guanzhong,Dezhou,Qingyang,Biyang,Guangling,Jinnan) in China was analyzed by technology of polymerase chain reaction based single strand conformation polymorphism(PCR-SSCP).Nineteen haplotypes with the percentage of 21.8% in 87 animals were obtained.The haplotype diversity was high as 0.834 and 0.904 for the Guanzhong donkeys and Jinnan donkeys,and low as 0.467 and 0.599 for the Qingyang donkeys and Dezhou donkeys,respectively,with that of 0.629 and 0.529 for the Biyang donkeys and Guangling donkeys respectively.It revealed the genetic diversity of different donkey breeds at molecular level and provided some bases for the breed resource evaluation and utilization of donkeys in China. |
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| Keywords: | donkey PCR-SSCP mtDNA D-Loop genetic diversity |
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