| 一株GPV的分离鉴定及致病性研究 |
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| 引用本文： | 饶桂波,邵洪泽,胡桂学,吴健敏.一株GPV的分离鉴定及致病性研究[J].家畜生态,2014(1):49-53. |
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| 作者姓名： | 饶桂波 邵洪泽 胡桂学 吴健敏 |
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| 作者单位： | [1]广西兽医研究所,广西南宁530001 [2]吉林省兽医科学研究所,吉林长春130062 [3]吉林农业大学,吉林长春130118 |
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| 摘 要： | 为了确定导致吉林省某地区10日龄雏鹅发病的病原，从病死雏鹅的肝脏中分离到一株病毒，根据病毒的电镜照片，动物回归试验及PCR鉴定，确定为鹅细小病毒。PCR产物测序后经BLAST比对显示，分离毒株与NCBI收录的GPVVP3基因同源性均在92％以上，与GPV／CH／HLJ02／08VP3基因的同源性最高，达99％。致病性研究表明，分离株的ELD50为10^-6.5／0．2mL，毒力较强，且该毒株能被GPV标准血清所中和。
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| 关 键 词： | 鹅细小病毒 分离鉴定 致病性 |
Isolation,Identification and Pathogenicity of a Strain of GPV |
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| RAO Gui-bo,SHAO Hong-ze,HU Gui-xue,WU Jian-min.Isolation,Identification and Pathogenicity of a Strain of GPV[J].Ecology of Domestic Animal,2014(1):49-53. |
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| Authors: | RAO Gui-bo SHAO Hong-ze HU Gui-xue WU Jian-min |
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| Institution: | 1 (1.Guangxi Veterinary Research Institute. Nanning Guangxi. 530001. China; 2. Jilin Institute of Veterinary Science. Changchun Jilin. 130062. China; 3. Jilin Agricultuγal University. Changchun Jilin. 130118. China) |
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| Abstract: | A strain of Goose parvirus isolated from the livers of dead goslings in an area of Jilin province was identified to be the pathogen of the 10-day-old goslings disease based on its electron micrograph, animal regression test and PCR results. A comparison through BLAST showed that the VP3 gene of the isolated strain had a homology of more than 92 % with those of the GPV included in NCB! and a highest homology with those of GPV /CH/HLJ02/08, which was up to 99 %. Pathogenicity research on it showed that its ELD50 is 10^-6.5/0.2 mL, a very strong virulence. And the strain can be neutralized by standard serum of the GPV. |
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| Keywords: | goose parvirus isolation and identification pathogenicity |
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