红景天多糖对幼鼠精原干细胞体外增殖的影响

为研究如何在体外获得更好的精原干细胞培养效果,以出生后6～8 d的小鼠为对象,应用两步酶消化法和差速贴壁法分离纯化精原干细胞.分别将浓度为100、200、300和400 ng/mL的红景天多糖加入以Sertoli细胞为饲养层的精原干细胞培养液中,以不添加红景天多糖组为对照,通过RT-PCR法和碱性磷酸酶（AP）染色鉴定细胞,MTT法研究红景天多糖对精原干细胞增殖的影响.RT-PCR和染色结果显示,分离得到的细胞为精原干细胞；MTT结果显示试验组比对照组细胞数量有显著增多（P＜0.05）,增殖率可达152％,且红景天多糖的最适添加量300 ng/mL.说明红景天多糖能显著促进小鼠精原干细胞的体外增殖.

Effects of Rhodiola Polysaccharides on Proliferation of Spermatogonial Stem Cells in vitro

In order to investigate the effect of Rhodiola polysaccharides on the proliferation of spermatogonial stem cells （SSCs） in vitro,newborn male mice at 6 to 8 days were adopted to separate and purify SSCs by two-step enzymes digestion and differential adhesion.Rhodiola Sachalinensis polysaccharides was added into the culture medium with different concentrations to identify the SSCs by RT-PCR testing and AP staining.The effect was tested by MTT after seeded on Sertoli cells which serve as layers and co-cultured with different concentrations of Rhodiola polysaccharides.The cells gained were SSCs by RT-PCR and AP staining.The result of MTT test showed that the amount of SSCs apparently increased （P＜0.05） after adding Rhodiola polysaccharides compared with the control,with an increase rate of 152％,and the optimal concentration was 300 ng/mL.The findings indicate that the Rhodiola polysaccharides can significantly promote the proliferation of spermatogonial stem cells in vitro.