| 犬瘟热病毒SH株N蛋白基因序列分析 |
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| 引用本文： | 张永霞,王金福,江立方,孟和.犬瘟热病毒SH株N蛋白基因序列分析[J].家畜生态,2009(2). |
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| 作者姓名： | 张永霞 王金福 江立方 孟和 |
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| 作者单位： | 上海农林职业技术学院;上海交通大学农业与生物学院; |
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| 基金项目： | 上海农林职业技术学院科研资助项目 |
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| 摘 要： | 根据GenBank中犬瘟热病毒(Canine distemper virus,CDV)N蛋白基因序列,设计合成1对特异性引物,以CDV SH株的总RNA为模板,RT-PCR扩增N蛋白基因,并进行克隆与序列分析。结果显示:CDV SH株N蛋白基因序列与CDV TN株、A75/17株、2 544/Han95株、98~2 654株、5 804株、Onderstepoort疫苗株的同源性分别为98.9%,97.6%,96.7%,96.9%,96.6%,93.9%;编码氨基酸的同源性分别为99.0%,98.7%,98.1%,97.9%,97.9%,96.9%。推测N蛋白是一个保守性非常强的结构蛋白。
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| 关 键 词： | 犬瘟热病毒 N蛋白 序列分析 |
The Sequence Analysis of N Protein Coding Gene of SH Strain of Canine Distemper Virus |
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| ZHANG Yong-xia,WANG Jin-fu,JIANG Li-fang,MENG He.The Sequence Analysis of N Protein Coding Gene of SH Strain of Canine Distemper Virus[J].Ecology of Domestic Animal,2009(2). |
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| Authors: | ZHANG Yong-xia WANG Jin-fu JIANG Li-fang MENG He |
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| Institution: | 1.Shanghai Vocational Technical College of Agriculture & Forestry;Shanghai 201600;China;2.College of Agriculture and biology;Shanghai Jiaotong University;Shanghai 200240;China |
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| Abstract: | According to the reported gene sequence of N protein of Canine Distemper Virus(CDV) in Gene Bank,a special pair of primers were designed to RT-PCR amplify N protein gene by using the total RNA of CDV SH strain as a template.The results showed that the homology of gene sequence between CDV SH strain and TN strain was 98.9%,A75/17 stain(97.6%),2544/Han95 strain(96.7%),98-2654 Strain(96.9%),5804 strain(96.6%) and Onderstepoort(93.3%) and the homology of coding amino acid between CDV SH strain and the others we... |
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| Keywords: | canine distemper virus N protein gene sequence |
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