小麦可溶性非淀粉多糖对肉仔鸡小肠粘膜二糖酶活性的影响

在体内、外研究了小麦可溶性非淀粉多糖(SNSP)对肉仔鸡小肠粘膜二糖酶活性的影响。取1日龄黄羽肉鸡105羽,随机分为3组,饲喂由玉米—豆粕、小麦—豆粕和玉米—豆粕加小麦SNSP提取物组成的3种日粮 ,分别于1、4、7、10、14、18和21日龄宰杀 ,取小肠粘膜 ,测定5种粘膜二糖酶的活性。体内试验结果表明 ,与玉米—豆粕日粮相比 ,小麦—豆粕日粮对肉仔鸡小肠粘膜乳糖酶、麦芽糖酶和纤维二糖酶活性抑制作用显著 (P<0.05)。玉米—豆粕加小麦SNSP提取物日粮对肉仔鸡小肠粘膜麦芽糖酶活性抑制作用显著 (P<0.05)。体外试验结果表明 ,小麦木聚糖显著降低乳糖酶、蔗糖酶、海藻糖酶和纤维二糖酶的活性 (P<0.05) ,极显著降低麦芽糖酶的活性 (P<0.01)。桦木木聚糖和小麦SNSP提取物分别显著降低蔗糖酶、海藻糖酶和纤维二糖酶的活性 (P<0.05) ,极显著降低麦芽糖酶的活性 (P<0.01)。研究结果表明 ,小麦SNSP无论在体内或在体外 ,均可抑制肉仔鸡小肠粘膜二糖酶活性。抑制小肠粘膜二糖酶活性是小麦SNSP抗营养作用的重要机制之一。

EFFECTS OF WHEAT SOLUBLE NON- STARCH POLYSACCHARIDES ON MUCOSA DISACCHRIDASE ACTIVITIES IN BROILERS

Effect of wheat soluble non-starch polysaccharides (SNSP) on intestinal mucosa disacchridase activities in yellow broilers was studied in vivo and in vitro.105 yellow broilers of 1 day age were separated into three groups randomly,fed three kinds of diets composed of corn-soybean meal,wheat-soybean meal and corn-soybean meal supplemented wheat soluble non-starch polysaccharides respectively.The birds were killed at 1,4,7,10,14,18 and 21 days respectively.Intestinal mucosa was taken and the activities of 5 varieties of disaccharidases in intestinal mucosa were determined.The in vivo experimental results showed that as compared with corn-soybean meal diet,feeding wheat-soybean meal diet significantly lowered the activities of lactase,maltase and cellobiase(P<0.05).Feeding corn-soybean meal diet supplemented wheat soluble non-starch polysaccharides significantly reduced activities of maltase(P<0.05).The in vitro experimental results showed that wheat arabinoxylan significantly decreased the activities of lactase,sucrase,trehalase,cellobiase(P<0.05),and maltase(P<0.01).Birchwood aradinoxylan and wheat soluble non-starch polysaccharides significantly decreased the activities of sucrase,trehalase,cellobiase(P<0.05) and maltase(P<0.01)respectively.The conclusion is that wheat SNSP both in vivo and in vitro trails lowers intestinal mucosa disacchridase activities in broilers,and that inhibiting intestinal mucosa disacchridase activities is one of important anti-nutritive mechanisms of wheat SNSP.