桦木酸对小鼠免疫器官抗氧化能力的影响

本试验旨在研究桦木酸(BA)对小鼠免疫器官抗氧化能力的影响.选取健康的昆明系小鼠28只,随机分为4组(每组7只),每天每只分别按每千克体重0、0.25、0.50、1.00 mg的剂量给予BA,BA混悬于0.2 mL1％可溶性淀粉溶液中.以灌胃方式给予,每天灌胃1次,连续灌胃14 d后,检测各组小鼠脾脏和胸腺中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GHS-Px)活性,丙二醛(MDA)含量以及总抗氧化能力(T-AOC).结果表明:BA可使小鼠脾脏和胸腺中SOD、GSH-Px活性以及T-AOC升高,MDA含量降低.其中,每千克体重灌胃0.50 mg BA能显著或极显著提高脾脏和胸腺中GSH-Px活性和T-AOC(P＜0.05或P＜0.01),显著或极显著降低胸腺和脾脏中MDA含量(P＜0.05或P＜0.01);每千克体重灌胃1.00 mg BA能显著或极显著提高脾脏和胸腺中SOD活性、T-AOC以及脾脏中GSH-Px活性(P＜0.05或P＜0.01),极显著降低胸腺和脾脏中MDA含量(P＜0.01).由此得出,BA能有效地增强小鼠免疫器官的抗氧化能力.

Effects of Betulinic Acid on Antioxidant Ability of Immune Organs in Mice

This experiment was conducted to investigate the effects of betulinic acid(BA) on antioxidant ability of immune organs in mice.A total of 28 KM mice were randomly divided into 4 groups with 7 mice in each group.The BA was administered once daily by gavage at dose levels of 0,0.25,0.50,and 1.00 mg per kg body weight for 14 days,and the BA was suspended in 0.2 mL of 1% soluble starch solution.The activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px),malondialdehyde(MDA) content and total antioxidant capacity(T-AOC) of spleen and thymus in mice were determined at the end of the experiment.The results showed as follows: the activities of SOD and GSH-Px,and T-AOC of spleen and thymus were increased,while the MDA content of spleen and thymus was decreased by BA.Thereinto,the GSH-Px activity and T-AOC of spleen and thymus were significantly increased(P<0.05 or P<0.01),while the MDA content of spleen and thymus was significantly decreased at the dose of 0.50 mg BA per kg body weight(P<0.05 or P<0.01);the activities of SOD and GSH-Px,and T-AOC of spleen and thymus were significantly increased(P<0.05 or P<0.01),while the content of MDA was significantly decreased at the dose of 1.00 mg BA per kg body weight(P<0.01).Therefore,BA can effectively improve the antioxidant ability of immune organs in mice.