妊娠后期营养限制对蒙古绵羊胎儿生物原胺类神经递质的影响

本试验旨在研究妊娠后期营养限制对蒙古绵羊胎儿生物原胺类神经递质的影响。选择健康、体况相近的蒙古绵羊18只,对其进行同期发情、配种后,从妊娠第90天开始,随机分为3个组:营养限制1组NG1组,n=6,代谢能(ME)=0.175 MJ/(kg BW 0.75·d)]、营养限制2组NG2组,n=6,ME=0.330 MJ/(kg BW 0.75·d)]和对照组CG组,n=6,ME=0.670 MJ/(kg BW 0.75·d)],在妊娠第140天时屠宰,取各组胎儿及其脑组织和血液。结果显示:NG1组胎儿的脑重较CG组显著增高(P<0.05),并且NG1组胎儿脑中5-羟色胺(5-HT)(P<0.01)、肾上腺素(EPI)(P<0.05)含量显著或极显著高于CG组,去甲肾上腺素(NA)的含量极显著低于CG组(P<0.01)。NG2组胎儿脑中5-HT含量极显著高于CG组(P<0.01),而NA(P<0.01)、多巴胺(DA)(P<0.05)含量显著或极显著低于CG组。另外,NG1组胎儿血浆中5-HT(P<0.01)、EPI(P<0.01)、NA(P<0.05)、DA(P<0.01)含量显著或极显著低于CG组;NG2组胎儿血浆中5-HT(P<0.01)、NA(P<0.01)、DA(P<0.05)含量显著或极显著低于CG组。由此得出,妊娠后期营养限制使得蒙古绵羊胎儿脑中5-HT、EPI含量升高和NA含量降低,血浆中5-HT、EPI、NA、DA含量降低。

Effects of Nutritional Restriction during Late Gestation on Biogenic Amine Neurotransmitters of Mongolian Sheep Fetus

The purpose of this study was to investigate the effects of nutritional restriction during late gestation on biogenic amine neurotransmitters of Mongolian sheep fetus.Eighteen Mongolian sheep with synchronization of estrus and conception were randomly divided into three groups at day 90 of gestation:nutritional restriction group 1NG1 group,n=6,metabolizable energy(ME)=0.175 MJ/(kg BW 0.75·d)],nutritional restriction group 2NG2 group,n=6,metabolizable energy(ME)=0.330 MJ/(kg BW 0.75·d)]and control groupCG group,n=6,metabolizable energy(ME)=0.670 MJ/(kg BW 0.75·d)].All sheep were slaughtered at 140 days of gestation,and the fetuses and their brain tissues and blood were sampled.The results showed that the fetal brain weight of NG1 group was significantly higher than that of CG group(P<0.05),and the contents of 5-hydroxytryptamine(5-HT)(P<0.01)and epinephrine(EPI)(P<0.05)were significantly or extremely significantly higher than those of CG group,while the content of noradrenaline(NA)was extremely significantly lower than that of CG group(P<0.01).The content of 5-HT in fetal brain of NG2 group was extremely significantly higher than that of CG group(P<0.01),while the content of NA(P<0.01)and dopamine(DA)(P<0.05)were significantly lower than those of CG group.In addition,the contents of 5-HT(P<0.01),EPI(P<0.01),NA(P<0.05)and DA(P<0.01)in fetal plasma of NG1 group were significantly or extremely significantly lower than those of CG group;the contents of 5-HT(P<0.01),NA(P<0.01)and DA(P<0.05)in fetal plasma of NG2 group were significantly or extremely significantly lower than those of CG group.In conclusion,nutritional restriction during late gestation makes the contents 5-HT,EPI increasing and the content of NA decreasing in Mongolian sheep fetal brain,as well as makes the contents of 5-HT,EPI,NA and DA decreasing in fetal brain.