饲粮中添加乳铁蛋白对早期断奶仔猪生长性能、肠道菌群及肠黏膜形态的影响

本试验旨在研究在饲粮中添加乳铁蛋白(lactoferrin,LF)对断奶仔猪生长性能、肠道菌群及肠黏膜形态的影响。试验选用96头体重相近的21日龄"杜×长×大"三元杂交断奶仔猪,随机分为4组,分别饲喂基础饲粮(对照组)、基础饲粮+250 mg/kg乳铁蛋白、基础饲粮+500 mg/kg乳铁蛋白和基础饲粮+750 mg/kg乳铁蛋白,每组4个重复,每个重复6头仔猪。正试期21 d。结果表明,与对照组和750 mg/kg乳铁蛋白组相比,饲粮中添加250和500 mg/kg乳铁蛋白可显著提高仔猪平均日增重(P<0.05);与对照组相比,不同水平乳铁蛋白可显著降低盲肠中大肠杆菌数量(P<0.05),其中添加500 mg/kg乳铁蛋白显著提高了盲肠、结肠中乳酸杆菌的数量(P<0.05),显著降低了结肠中大肠杆菌数量(P<0.05);添加250和500 mg/kg乳铁蛋白极显著提高了十二指肠、空肠、回肠的绒毛高度/隐窝深度比值(P<0.01)。结果提示,饲粮中添加乳铁蛋白可刺激肠道有益菌生长、降低有害菌增殖,从而改善肠道功能,具有提高仔猪生长性能的作用,本试验条件下乳铁蛋白的适宜添加量为250 mg/kg。

Effects of Lactoferrin Supplementation on Growth Performance, Intestinal Microflora and Mucosal Morphology of Early Weaned Piglets

This experiment was conducted to investigate the effects of lactoferrin(LF)on growth performance,intestinal microflora and mucosal morphology in early weaned piglets.Ninety-six piglets weaned at 21 days of age were randomly divided into four groups with four replicates in every group and 6 piglets in every replicate.The four groups were basal diet group,basal diet+250 mg/kg LF group,basal diet+500 mg/kg LF group and basal diet+750 mg/kg LF group,respectively.The experiment lasted for 21 days.The results showed that compared with the control and 750 mg/kg LF group,diets supplemented with 250 and 500 mg/kg LF could significantly improve average daily gain(P<0.05);compared with the control group,LF could reduce the sum of Escherichia coli in caecum(P<0.05).The supplementation of 500 mg/kg LF was significantly increased Lactobacillus number in caecum and colon(P<0.05)as well as significantly reduced Escherichia coli number in colon(P<0.05).The supplementation of 250 and 500 mg/kg LF were extremely significantly increased villus height to crypt depth ratio in duodenum,jejunum and ileum(P<0.01).The results indicate that supplementation of LF can improve the growth performance and regulate intestinal microflora as well as mucosal morphology.The optimum supplemental level of LF is 250 mg/kg under this experimental condition.