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用荧光标记细菌法研究丝兰提取物对瘤胃原虫对细菌吞噬的影响
引用本文:曹恒春,王洪荣,王梦芝,李国祥,徐爱秋.用荧光标记细菌法研究丝兰提取物对瘤胃原虫对细菌吞噬的影响[J].动物营养学报,2009,21(3).
作者姓名:曹恒春  王洪荣  王梦芝  李国祥  徐爱秋
作者单位:扬州大学动物科学与技术学院,扬州,225009
摘    要:采用荧光染色标记细菌(fluorescence labeled bacteria,FLB)技术,研究山羊瘤胃中原虫对细菌的吞噬速率.试验选用3头装有永久瘘管的徐淮山羊作为瘤胃液供体,采用体外培养法研究在精粗比为1:9的情况下,探讨不同丝兰提取物水平(A组0 mg/L、B组35 mg/L、C组70 mg/L)对瘤胃原虫吞噬细菌速率的影响.荧光镜检与回归分析结果表明:原虫吞噬细菌的速率:A组:339.9 cells/(cell·h);B组:314.7 cells/(cell·h);C组:339.9 cells/(cell·h).结果表明荧光标记细菌技术能够应用于瘤胃原虫吞噬细菌速率的研究.动物营养学报,2009,21(3):417-422]中文全文见<动物营养学报>网站(www.ChinaJAN.com)中文版2009年21卷3期]

关 键 词:荧光染色标记细菌  吞噬速率  细菌N

Effects of Yucca Schidigera on Determination of Bacterial Predation Rate by Rumen Protozoa Based on a Fluorescence-labeled Technique
CAO Heng-chun,WANG Hong-rong,WANG Meng-zhi,LI Guo-xiang,XU Ai-qiu.Effects of Yucca Schidigera on Determination of Bacterial Predation Rate by Rumen Protozoa Based on a Fluorescence-labeled Technique[J].Acta Zoonutrimenta Sinica,2009,21(3).
Authors:CAO Heng-chun  WANG Hong-rong  WANG Meng-zhi  LI Guo-xiang  XU Ai-qiu
Abstract:Research on the bacterial predation rate by rumen protozoa was carried out using a technique of fluorescence-labeled bacteria in culture in vitro incubation. Three Xuhuai goats fitted with rumen cannula were used to determine the effects of yucca schidigera extract (YSE) at 0 (group A), 35 (group B) and 70 mg/L (group C) on the determination of bacterial predation rate by rumen protozoa when dietary concentrate to forage ratio was set to 1 : 9 in the culture. The results showed that the predation rates of rumen protozoa were 339. 9 cells/(cell·h) in group A, 314. 7 cells/(cell·h) in group B, 339. 9 cells/(cell·h) in group C, respectively. It was concluded that the fluorescence-labeled bacteria technique would be a potential option for determination of bacterial predation rate by rumen protozoa.
Keywords:Fluorescence-labeled bacteria  Predation rate  Bacterial-N
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