| 传染性法氏囊病病毒VP2基因真核表达载体的构建及初步表达 |
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| 引用本文： | 鲁宏伟,闫强,陈明勇,王宾.传染性法氏囊病病毒VP2基因真核表达载体的构建及初步表达[J].动物医学进展,2006,27(6):72-74. |
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| 作者姓名： | 鲁宏伟 闫强 陈明勇 王宾 |
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| 作者单位： | 1. 中国农业大学动物医学院,北京100094
2. 中国农业大学农业生物技术国家重点实验室,北京100094 |
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| 摘 要： | 根据GenBank已登录的传染性法氏囊病病毒VP2基因序列,设计1对特异引物,应用反转录-聚合酶链反应技术从标准毒株B87中扩增了VP2基因,将其克隆到proVAX载体上,构建了proVAX-VP2真核表达载体,在脂质体介导下转染Hela细胞,用RT-PCR方法从转录水平证实VP2在Hela细胞中有特异性表达。
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| 关 键 词： | 传染性法氏囊病病毒 VP2基因 真核表达 |
| 文章编号： | 1007-5038（2006）06-0072-03 |
| 收稿时间： | 2006-03-27 |
| 修稿时间： | 2006年3月27日 |
Construction and Expression of Eukaryotic Expression Plasmid of VP2 Gene of Infectious bursal disease virus |
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| LU Hong-wei,YAN Qiang,CHEN Ming-yong,WANG Bin.Construction and Expression of Eukaryotic Expression Plasmid of VP2 Gene of Infectious bursal disease virus[J].Progress In Veterinary Medicine,2006,27(6):72-74. |
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| Authors: | LU Hong-wei YAN Qiang CHEN Ming-yong WANG Bin |
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| Institution: | 1. College of Veterinary Medicine, China Agricultural University, Beijing , 100094, China ; 2. State Key Laboratory for Agricultural Biotechnology, China Agricultural University ,Beijing, 100094 ,China |
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| Abstract: | According to the published sequence of VP2 gene of IBDV, a pair of specific primers was designed and synthesized.The VP2 gene was amplified by RT-PCR method from B87 strain,and cloned into proVAX Vector.Eukaryotic Vector proVAX-VP2 was constructed and transinfected into Hela cell induced by Liprofectamine~(TM)2000.The expression of VP2 gene was primarily proved by RT-PCR. |
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| Keywords: | Infectious bursal disease virus VP2 gene eukaryotic expression |
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