| 共表达NDV F和IBDV VP0基因重组鸡痘病毒遗传稳定性研究 |
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| 引用本文： | 隋胜波,王军一,徐敬龙,金扩世,胡仲明,金宁一.共表达NDV F和IBDV VP0基因重组鸡痘病毒遗传稳定性研究[J].动物医学进展,2007(2). |
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| 作者姓名： | 隋胜波 王军一 徐敬龙 金扩世 胡仲明 金宁一 |
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| 作者单位： | 青岛动物园 山东青岛266003(隋胜波)，山东临沂畜牧局 山东临沂276004(王军一)，军事医学科学院军事兽医研究所 吉林长春130062(徐敬龙，金扩世，胡仲明，金宁一) |
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| 基金项目： | 总后勤部青年基金项目(01Q127)，澳利集团有限公司资助项目 |
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| 摘 要： | 将共表达NDV F和IBDV VP0基因重组鸡痘病毒(重组鸡痘病毒vFV282)接种10日龄~11日龄SPF鸡胚,连续传10代,分别对第5、8、10代传代毒进行病毒毒价测定和PCR鉴定;重组鸡痘病毒vFV282接种鸡胚成纤维细胞,分别对第1、5、10、15、20、25、30代传代毒进行病毒毒价测定和PCR鉴定;重组鸡痘病毒vFV282翅翼刺种于鸡体上,连续传8代,对第3、5、8代传代毒进行病毒毒价测定和PCR鉴定。结果表明,重组鸡痘病毒vFV282在SPF鸡胚上传10代、在SPF鸡胚成纤维细胞传30代、在鸡体传8代后,其毒力未返强,F基因和VP0基因未发生缺失和变异。
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| 关 键 词： | NDVF基因 鸡痘病毒 IBDV VP0基因 遗传稳定性 毒价测定 |
The Genetic Stability of Recombinant Fowlpox virus Coexpressing NDV F Gene and IBDV VP0 Gene |
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| SUI Sheng-bo.The Genetic Stability of Recombinant Fowlpox virus Coexpressing NDV F Gene and IBDV VP0 Gene[J].Progress In Veterinary Medicine,2007(2). |
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| Authors: | SUI Sheng-bo |
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| Institution: | SUI Sheng-bo~ |
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| Abstract: | In order to evaluate the genetic stability of vFV282, we examined the F gene of NDV and VP0 gene of IBDV carried by vFV282.First,vFV282 was cultivated on SPF embryoes of chicken 10 passages continuously.Then,passage 5th,8th,10th~()were identified by PCR and their TCID_(50) of vFV282 were examined.Secondly,vFV282 was cultivated on CEF 30 passages continuously.And passage 1st,5th,10th,15th,20th,25th,30th were identified.Finally,vFV282 was cultivated 8 passages on chickens continuously,and 3rd,5th,8th~()were identified.The results indicated that the variation and loss of VP0 and F genes did not happen,and the TCID_(50) did not become strong. |
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| Keywords: | NDV F gene Fowlpox virus IBDV VP0 gene genetic stability examination on TCID_(50) |
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