| 小反刍兽疫病毒N基因的原核表达 |
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| 引用本文： | 阮洋,秦峻岭,高玉伟,孙玮,张涛,杨玉姣,杨松涛,王承宇,王铁成,黄耕,夏咸柱.小反刍兽疫病毒N基因的原核表达[J].动物医学进展,2011,32(3):21-25. |
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| 作者姓名： | 阮洋 秦峻岭 高玉伟 孙玮 张涛 杨玉姣 杨松涛 王承宇 王铁成 黄耕 夏咸柱 |
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| 作者单位： | 阮洋,RUAN Yang(吉林农业大学动物科学技术学院,吉林长春,130118;军事医学科学院军事兽医研究所,吉林省人兽共患病预防与控制重点实验室,吉林长春,130062);秦峻岭,孙玮,杨玉姣,QIN Jun-ling,SUN Wei,YANG Yu-jiao(军事医学科学院军事兽医研究所,吉林省人兽共患病预防与控制重点实验室,吉林长春,130062;吉林大学畜牧兽医学院,吉林长春,130062);高玉伟,张涛,杨松涛,王承宇,王铁成,黄耕,GAO Yu-wei,ZHANG Tao,YANG Song-tao,WANG Cheng-yu,WANG Tie-cheng,HUANG Geng(军事医学科学院军事兽医研究所,吉林省人兽共患病预防与控制重点实验室,吉林长春,130062);夏咸柱,XIA Xian-zhu(吉林农业大学动物科学技术学院,吉林长春,130118;军事医学科学院军事兽医研究所,吉林省人兽共患病预防与控制重点实验室,吉林长春,130062;吉林大学畜牧兽医学院,吉林长春,130062) |
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| 基金项目： | 公益性行业科研专项项目 |
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| 摘 要： | 目的是表达出小反刍兽疫病毒的核蛋白,并鉴定其活性.根据GenBank发表的小反刍兽疫病毒(PPRV)Nigeria 75/1株N基因序列,对其进行基因优化并合成.设计引物,利用PCR的方法扩增PPRV-N基因,将该基因片段定向克隆到原核表达载体pET-28a(+)中,构建原核表达栽体pET-28a-N.阳性质粒转化原核...
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| 关 键 词： | 小反刍兽疫病毒N基因 原核表达 SDS-PAGE电泳 Western-blot |
The Prokaryotic Expression of N Gene from Peste Des Petits Ruminants Virus |
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| RUAN Yang,QIN Jun-ling,GAO Yu-wei,SUN Wei,ZHANG Tao,YANG Yu-jiao,YANG Song-tao,WANG Cheng-yu,WANG Tie-cheng,HUANG Geng,XIA Xian-zhu.The Prokaryotic Expression of N Gene from Peste Des Petits Ruminants Virus[J].Progress In Veterinary Medicine,2011,32(3):21-25. |
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| Authors: | RUAN Yang QIN Jun-ling GAO Yu-wei SUN Wei ZHANG Tao YANG Yu-jiao YANG Song-tao WANG Cheng-yu WANG Tie-cheng HUANG Geng XIA Xian-zhu |
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| Institution: | RUAN Yang1,2,QIN Jun-ling2,3,GAO Yu-wei2,SUN Wei2,ZHANG Tao2,YANG Yu-jiao2,YANG Song-tao2,WANG Cheng-yu2,WANG Tie-cheng2,HUANG Geng2,XIA Xian-zhu1,3(1.College of Animal Science and Technology,Jilin Agricultural University,Changchun,Jilin,130118,China,2.Institute of Military Veterinary,Academy of Military Medical Science,Key Laboratory of Jilin Province for Zoonosis Prevention and Control,130062,3.College of Animal Science and Veterinary Medicine,Jilin University,1... |
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| Abstract: | To acquire the N protein of peste des petits ruminants virus(PPRV) by expressing N gene in prokaryotic system,the complete length of N gene from peste des petits ruminants virus was amplified by PCR using a pair of specific primers designed according to the sequences in GenBank,and the PCR products were cloned into prokaryotic expression vector pET28a(+) to obtain the prokaryotically expressed plasmid pET-28a-N.The target gene was then expressed in E.coli Transetta(DE3) cells inducted with IPTG,and the expr... |
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| Keywords: | Western-blot |
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