| 羔羊外周血单个核细胞转化MTT比色法最佳检测条件的筛选 |
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| 引用本文： | 张冰冰,赵魁,贺文琦,王栋,臧德跃,陈克研,王改丽,高丰.羔羊外周血单个核细胞转化MTT比色法最佳检测条件的筛选[J].动物医学进展,2011,32(3):65-68. |
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| 作者姓名： | 张冰冰 赵魁 贺文琦 王栋 臧德跃 陈克研 王改丽 高丰 |
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| 作者单位： | 吉林大学畜牧兽医学院,吉林长春,130062 |
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| 基金项目： | 吉林省科技发展计划重点项目 |
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| 摘 要： | 为了建立羔羊外周血单个核细胞转化检测的MTT比色法,对影响MTT比色法的3个主要因素(包括刺激原、细胞浓度、培养时间)进行了探索性的研究.结果表明,MTT法检测的最佳条件为:植物血凝素(PHA)浓度为10 pg/mL,细胞密度为1×107个/mL;或者刀豆蛋白A(ConA)浓度为40μg/mL,细胞密度为2×107个/...
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| 关 键 词： | 羔羊 外周血单个核细胞 MTT |
Study on Optimual Condition of MTT in PBMC Transformation |
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| ZHANG Bing-bing,ZHAO Kui,HE Wen-qi,WANG Dong,ZANG De-yue,CHEN Ke-yan,WANG Gai-li,GAO Feng.Study on Optimual Condition of MTT in PBMC Transformation[J].Progress In Veterinary Medicine,2011,32(3):65-68. |
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| Authors: | ZHANG Bing-bing ZHAO Kui HE Wen-qi WANG Dong ZANG De-yue CHEN Ke-yan WANG Gai-li GAO Feng |
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| Institution: | ZHANG Bing-bing,ZHAO Kui,HE Wen-qi,WANG Dong,ZANG De-yue,CHEN Ke-yan,WANG Gai-li,GAO Feng(College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jilin,130062,China) |
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| Abstract: | Three major factors which affected the PBMC including concertrations of stimulus and lymphocyte and culture time were studied.The test showed the best condition of reaction was 10 μg/mL PHA,1×107/mL cell concentration;or 40 μg/mL ConA,2×107/mL cell concentration,reaction condition was 37 ℃ incubated 48 hours. |
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| Keywords: | MTT |
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