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副猪嗜血杆菌的分离鉴定与药敏试验
引用本文:易顺华,陈进喜,蒋碧桂.副猪嗜血杆菌的分离鉴定与药敏试验[J].动物医学进展,2009,30(9):41-44.
作者姓名:易顺华  陈进喜  蒋碧桂
作者单位:1. 广西大学动物科学技术学院,广西南宁,530005
2. 广西北斗星动物保健品有限公司,广西南宁,530005
摘    要:对一例临床症状、病理剖检变化疑似副猪嗜血杆菌病的仔猪病料进行实验室诊断,进行了细菌培养特性和生化特性等鉴定,初步怀疑为副猪嗜血杆菌,根据副猪嗜血杆菌的16 S rRNA基因设计特异性引物进行PCR扩增鉴定,结果表明,分离菌为副猪嗜血杆菌.药敏试验显示,该分离菌对阿米卡星、头孢氨苄、新霉素高度敏感.

关 键 词:副猪嗜血杆菌  分离  鉴定  药敏试验

Identification and Drug Sensitivity Test of Haemophilus parasuis Isolate
YI Shun-hua,CHEN Jin-xi,JIANG Bi-gui.Identification and Drug Sensitivity Test of Haemophilus parasuis Isolate[J].Progress In Veterinary Medicine,2009,30(9):41-44.
Authors:YI Shun-hua  CHEN Jin-xi  JIANG Bi-gui
Institution:YI Shun-hua1,CHEN Jin-xi1,JIANG Bi-gui2(1.College of Animal Science and Technology,Guangxi University,Nanning,Guangxi,530005,China,2.Guangxi Wain Animal Drugs Co.,Ltd,China)
Abstract:A pig whose clinical symptomas looked like Haemophilus parasuis(Hps) infection was checked by lab technologies.The strain was suspected to be Hps according to its culture characteristics and biochemical assay.A pair of primers were designed according to the sequence of the 16S rRNA gene of Hps,then this strain was identified as Hps by PCR.Drug sensitivity test showed that Hps was sensitive to amikacin,cefalexin and neomgcin.
Keywords:Haemophilus parasuis  isolation and identification  drug sensitivity test  
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