猪链球菌2型湖南分离株多重耐药性及相关耐药基因研究

基因突变和基因转移是细菌耐药性产生和存在的重要内因,检测与抗生素耐药性相关的基因具有重要的意义.试验选取25份猪链球菌2型湖南分离株对16种抗生素进行药敏试验,检测菌株耐药性;选出了23株有红霉素抗性的菌株,用PCR检测其erm(B)基因.结果显示,猪链球菌湖南分离株对红霉素、四环素、万古霉素和克林霉素具有高耐药性,在23株红霉素抗性菌株中有18株存在erm(B)基因,由erm(B)基因产生的红霉素耐药菌株占到其中的78.3%.因此,erm(B)基因是链球菌2型湖南分离株对红霉素耐药的主要抗性决定基因.

Study on Multi-drug Resistance and Relative Resistance Gene of Streptococcus suis 2 Isolates in Hunan

Streptococcus suis was a well-recognized worldwide swine pathogen of emerging clinical significance in most countries with intensive swine industry.Gene mutation and transfer are the major internal cause to drug resistance.We selected 25 Streptococcus suis 2 strains isolated in Hunan to test their drug resistance in vitro.Then 23 strains of these Streptococcus suis 2 which resisted to erythromycin were tested for resistance gene erm(B) by PCR.The results showed that resistance to erythromycin,tetracycline,c...