噬菌体表面展示技术的发展及应用

随着噬菌体展示技术自身建库、筛选方法等方面的进一步改进和完善,目前已被广泛应用于生命科学的许多领域,特别是噬菌体表面展示技术在蛋白质相互作用方面的成功应用,为筛选新的结合蛋白提供了一种简单、有效的手段,还为功能基因组学的研究提供了一个新的方法。该技术还在药物研制和疾病机理方面显示出很大的应用潜力,目前已成为筛选对人类疾病有诊断和治疗价值的生物活性分子的重要手段,并已广泛用于癌症、艾滋病、心血管病、组织器官移植、神经性疾病等领域药物的研究和开发。

Advance and Application of Phage Display Techniques

With the development in methods of construction and screening, phage display techniques for functional peptides as well as functional genomics provide a simple and powerful tool of screening new proteins and have been extensively used in many fields of bio-science. In addition, because of advantageousness in studies of drug design and pathology,they have been utilized in screening of molecules which are useful in human diseases' diagnosis and treatment, such as cancer, AIDS, transplant and so on. Here, their applicable prospects and studies in protein interaction, drug design, pathogenic mechanism, etc. are discussed.