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肉鸡鸡白痢沙门菌的分离鉴定
引用本文:王春丽,姜北宇,曹峰子,陈小玲,张培君,王宏俊.肉鸡鸡白痢沙门菌的分离鉴定[J].动物医学进展,2010,31(12).
作者姓名:王春丽  姜北宇  曹峰子  陈小玲  张培君  王宏俊
作者单位:1. 北京市农林科学院畜牧兽医研究所,北京,100097;吉林农业大学动物科学技术学院,长春吉林,130118
2. 北京市农林科学院畜牧兽医研究所,北京,100097
3. 北京市农林科学院畜牧兽医研究所,北京,100097;河北农业大学动物科技学院,保定河北,073000
基金项目:北京市农林科学院青年基金项目(20090805)
摘    要:2009年5月,北京郊区某肉鸡养殖户的2 000只7日龄雏鸡出现拉白色稀粪,肝脏有黄色结节等症状和病变的疾病。为探索该病病因及制定有效防治措施,对病鸡样品进行了细菌学检验。通过细菌分离、生化试验、PCR及16 S rRNA基因测序鉴定出1株鸡白痢沙门菌。该分离株感染试验鸡能复制出与其临床表现一致的病例并能引起死亡。表明鸡白痢沙门菌是危害北京郊区该养殖户肉鸡的病原。

关 键 词:肉鸡  鸡白痢沙门菌  实验室检验  

Isolation and Identification of Salmonella pullorum from Broiler
WANG Chun-li,JIANG Bei-yu,CAO Feng-zi,CHEN Xiao-ling,ZHANG Pei-jun,WANG Hong-jun.Isolation and Identification of Salmonella pullorum from Broiler[J].Progress In Veterinary Medicine,2010,31(12).
Authors:WANG Chun-li  JIANG Bei-yu  CAO Feng-zi  CHEN Xiao-ling  ZHANG Pei-jun  WANG Hong-jun
Institution:WANG Chun-li1,2,JIANG Bei-yu1,CAO Feng-zi1,3,CHEN Xiao-ling1,ZHANG Pei-jun1,WANG Hong-jun1(1.Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing,100097,China,2.College of Animal Science and Technology,Jilin Agricultural University,Changchun,Jilin,130118,3.College of Animal Science and Technology,Hebei Agricultural University,Baoding,Hebei,073000,China)
Abstract:In May 2009,an enteric outbreak showing blanc-loose stool and flavi-tubercle type of lesion on chick liver occurred among 2000 heads of 7-day-old to 10-day-old chicks in a commercial broiler farm in Beijing suburban area.To identify the pathogen and establish control measures,samples were taken from sick birds and bacteriological examination was performed.Through bacterial isolation,biochemical tests,PCR,16 S rRNA sequencing,a Salmonella pullorum was identified.The chicks infected with the isolate showed si...
Keywords:broiler  Salmonella pullorum  laboratory test  
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