| 乳胶凝集试验快速检测副猪嗜血杆菌 |
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| 引用本文： | 曹芸,陈品,何启盖,张坤,李文洁,柴艳东.乳胶凝集试验快速检测副猪嗜血杆菌[J].动物医学进展,2009,30(12):8-13. |
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| 作者姓名： | 曹芸 陈品 何启盖 张坤 李文洁 柴艳东 |
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| 作者单位： | 华中农业大学农业微生物学国家重点实验室,湖北武汉,430070 |
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| 基金项目： | 国家十一·五科技支撑计划项目，教育部新世纪优秀人才资助计划 |
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| 摘 要： | 利用原核表达并经过纯化的副猪嗜血杆菌TbpB N端蛋白及人工合成的特异性多肽制备单克隆抗体,经碳化二亚胺(EDC)将纯化后的单克隆抗体IgG与羧化乳胶共价偶联,并对偶联乳胶的IgG含量及偶联时间等条件进行合理选择,建立了快速检测副猪嗜血杆菌的乳胶凝集试验方法.利用LAT方法检测148株副猪嗜血杆菌疑似菌株,参考16 S rRNA-PCR方法的结果,符合率为83.1%,且致敏乳胶不与临床常见菌株发生凝集反应.结果表明,此检测方法具有操作简便、快速、特异性高的特点,便于在基层推广使用,为副猪嗜血杆菌的诊断和有效防治提供了参考和依据.
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| 关 键 词： | 副猪嗜血杆菌 转铁结合蛋白 单克隆抗体 羧化乳胶 乳胶凝集 |
A Latex Agglutination Test for Detection of Haemophilus Parasuis |
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| CAO Yun,CHEN Pin,HE Qi-gai,ZHANG Kun,LI Wen-jie,CAI Yan-dong.A Latex Agglutination Test for Detection of Haemophilus Parasuis[J].Progress In Veterinary Medicine,2009,30(12):8-13. |
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| Authors: | CAO Yun CHEN Pin HE Qi-gai ZHANG Kun LI Wen-jie CAI Yan-dong |
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| Institution: | CAO Yun,CHEN Pin,HE Qi-gai,ZHANG Kun,LI Wen-jie,CAI Yan-dong (Laboratory of Animal Viology,Huazhong Agricultural University,Wuhan,Hubei,430070,China) |
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| Abstract: | A Latex Agglutination Test (LAT) was developed for quick detection of Haemophilus parasuis. Recombinant TbpB-GST protein of Haemophilus parasuis was obtained and purified, as well as a unique 91-bp polypeptide within TbpB N-terminal domain from position -226 to -318 was synthesized. Afterwards, two groups of six to eight-week-old female Balb/c mice each was immunized with the recombinant fusion protein and the peptide conjugated with KLH (keyhole limpet hemocyanin). Two individual monoclonal antibodies IgGs... |
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| Keywords: | Haemophilus parasuis TbpB monoclonal antibody carboxylated latex LAT |
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