| 布鲁菌OMP25基因重组山羊痘病毒的构建 |
| |
| 引用本文： | 魏凤,郭志儒,陈创夫,乔军.布鲁菌OMP25基因重组山羊痘病毒的构建[J].动物医学进展,2008,29(4):6-9. |
| |
| 作者姓名： | 魏凤 郭志儒 陈创夫 乔军 |
| |
| 作者单位： | 石河子大学动物科技学院,新疆石河子,832000 |
| |
| 摘 要： | 将羊种布鲁菌外膜蛋白25(OMP25)基因插入到山羊痘病毒转移载体PGM-TK-I1L-GPT1启动子I1L的下游,构建重组山羊痘病毒转移载体。用脂质体转染法将重组转移载体与山羊痘病毒疫苗株G14-STV44-55共转染绵羊成纤维细胞,在细胞内同源重组。通过霉酚酸药物筛选、纯化,经PCR鉴定获得了重组山羊痘病毒。
|
| 关 键 词： | 羊种布鲁菌 外膜蛋白25基因 重组山羊痘病毒 |
| 文章编号： | 1007-5038(2008)04-0006-04 |
| 修稿时间： | 2007年11月25 |
Construction of the Recombinant Goatpoxvirus Expressing OMP25 Gene of B.melitensis |
| |
| WEI Feng,GUO Zhi-ru,CHEN Chuang-fu,QIAO Jun.Construction of the Recombinant Goatpoxvirus Expressing OMP25 Gene of B.melitensis[J].Progress In Veterinary Medicine,2008,29(4):6-9. |
| |
| Authors: | WEI Feng GUO Zhi-ru CHEN Chuang-fu QIAO Jun |
| |
| Abstract: | To construct the Recombinant goatpoxvirus expressing keywrod's ontside membrance protein(OMP25) gene of B.melitensis,the OMP25 gene was inserted into the downstream of promotor I1L of goatpoxvirus(GPV)transfer vector PGM-TK-I1L-GPT1.Then the recombinant transfer vector was transfected into sheep fibroblast cells infected with GPV vaccine strain G14-STV44-55 by lipofectamine.The transfected sheep fibroblast cells were conducted by mycophenolic acid(MPA)to screen and purify recombinant goatpoxvirus(RGPV).Recombinant goatpoxvirus was successfully obtained and identified by PCR. |
| |
| Keywords: | B melitensis outside membrance protein 25 gene recombinant Goatpoxvirus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
