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| 山羊痘病毒诱导BHK-21细胞凋亡的检测 | |
| 引用本文： | 程振涛,岳筠,周碧君,王开功,文明,李永明,许乐仁,朱时杰.山羊痘病毒诱导BHK-21细胞凋亡的检测[J].动物医学进展,2011,32(4):49-54. |
| 作者姓名： | 程振涛 岳筠 周碧君 王开功 文明 李永明 许乐仁 朱时杰 |
| 作者单位： | 1. 贵州大学动物疫病研究所,贵州贵阳550025;贵州大学,贵州贵阳550025;贵州省动物疫病研究室,贵州贵阳550025 2. 贵州大学,贵州贵阳,550025 3. 贵州大学动物疫病研究所,贵州贵阳550025;贵州大学,贵州贵阳550025 |
| 基金项目： | 国家自然科学基金，贵州省优秀科技教育人才省长专项基金，贵州省农业委员会科技计划项目，贵州大学大学生创新性实验计划项目 |
| 摘 要： | 为验证山羊痘病毒诱导BHK-21细胞出现凋亡现象的存在,分别采用HE染色、凋亡试剂盒检测、流式细胞仪检测和DNA Ladder检测,对山羊痘病毒感染的BHK21细胞培养物进行细胞凋亡检测.不同方法检测结果均显示,山羊痘病毒感染BHK-21细胞组相同时间细胞凋亡数明显高于对照组.表明山羊痘病毒可诱导BHK-21细胞发生... |
| 关 键 词： | 山羊痘病毒 BHK-21细胞 细胞凋亡 检测 |
Detection of BHK-21 Cell Apoptosis Induced by Goatpox Virus |
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| CHENG Zhen-tao,YUE Jun,ZHOU Bi-jun,WANG Kai-gong,WEN Ming,LI Yong-ming,XU Le-ren,ZHU Shi-jie.Detection of BHK-21 Cell Apoptosis Induced by Goatpox Virus[J].Progress In Veterinary Medicine,2011,32(4):49-54. | |
| Authors: | CHENG Zhen-tao YUE Jun ZHOU Bi-jun WANG Kai-gong WEN Ming LI Yong-ming XU Le-ren ZHU Shi-jie |
| Institution: | 1.Institute of Animal Disease,Guizhou University,Guiyang,Guizhou,550025,China;2.College of Animal Science,Guizhou University,Guiyang,Guizhou,550025,China;3.Laboratory for Animal Epidemic Disease of Guizhou,Guiyang,Guizhou,550025,China) |
| Abstract: | In order to identify the BHK-21 cell apoptosis induced by Goatpox virus,the HE staining method,apoptosis detection kit,flow cytometry and DNA Ladder test were used to detect the BHK-21 cell cultures which infected with goat pox virus.The results of different test method showed the same that the apoptosis cell percentages in experimental groups were higher than that in the control group.The research results demonstrated that the Goatpox virus can cause the BHK-21 cell apoptosis. |
| Keywords: | Goatpox virus BHK-21 cell cell apoptosis detection |
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