| 犬细小病毒的分离鉴定 |
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| 引用本文： | 杜强,邱薇,范泉水,刘华,李作生,郑颖,张富强.犬细小病毒的分离鉴定[J].动物医学进展,2009,30(3). |
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| 作者姓名： | 杜强 邱薇 范泉水 刘华 李作生 郑颖 张富强 |
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| 作者单位： | 1. 云南农业大学动物科技学院,云南昆明,650201
2. 成都军区疾控中心军事医学研究所,云南昆明,650032 |
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| 摘 要： | 用猫肾(F81)细胞从沈阳某养犬场病死犬的肠内容物中,分离到1株细小病毒.根据犬细小病毒(CPV)VP2基因的核苷酸序列设计合成了两对特异性引物,对分离的病毒株进行PCR扩增,分别得到846 bp和815 bp的2个片段,PCR产物经纯化后测序,测序结果与GenBank中已发表的CPV参考株PLI-IV(typeFPV)、CPV-b(type2)、V154(type2a)、LCPV-V204(type2b)、LCPV-V139(type2c(a))和LCPV-V203(type2c(b))的VP2基因序列相比较,根据分析比较的数据结果,确定此株细小病毒为CPV-2a亚型.
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| 关 键 词： | 犬细小病毒 分离 鉴定 |
Isolation and Identification of a Canine parvovirus Strain |
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| DU Qiang,QIU Wei,FAN Quan-shui,LIU Hua,LI Zuo-sheng,ZHENG Ying,ZHANG Fu-qiang.Isolation and Identification of a Canine parvovirus Strain[J].Progress In Veterinary Medicine,2009,30(3). |
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| Authors: | DU Qiang QIU Wei FAN Quan-shui LIU Hua LI Zuo-sheng ZHENG Ying ZHANG Fu-qiang |
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| Institution: | 1.College of Animal Science;Yunnan Agricultural University;Kunming;Yunan;650201;China;2.The Military Medical Institute of Cheng-du Military Area;Yunnan;650032;China |
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| Abstract: | A strain of canine parvovirus was isolated from the feces of a dead dog. Two pairs of primers of the VP2 gene were designed according to the sequence of CPV published in GenBank, two gene fragments about 800 bp were amplified in this study. After sequencing and analysing the sequence of these fragments,the result was compared with GenBank CPV reference strains PLI-IV(typeFPV), CPV-b(type2),V154(type2a),LCPV-V204(type2b) LCPV-V139(type2c(a))and LCPV-V203(type2c(b)).This strain was defined as a CPV-2a hypotyp... |
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| Keywords: | Canine parvovirus isolation identification |
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