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宁夏部分地区鸡大肠埃希菌的分离与血清型鉴定
引用本文:张燕飞,王东,王玉炯,何玉龙,王建波,蒲建明,丁林军,扈登强.宁夏部分地区鸡大肠埃希菌的分离与血清型鉴定[J].动物医学进展,2012,33(5):119-121.
作者姓名:张燕飞  王东  王玉炯  何玉龙  王建波  蒲建明  丁林军  扈登强
作者单位:1. 宁夏大学西部特色生物资源保护与利用教育部重点实验室,宁夏银川,750021
2. 银川市农业局,宁夏银川,750001
3. 吴忠市畜牧局,宁夏吴忠,751100
4. 宁夏博奥药业有限公司,宁夏银川,750021
基金项目:国家自然科学基金,宁夏大学自然科学基金
摘    要:为调查宁夏地区鸡大肠杆菌病的流行情况,采集宁夏4个地区的103个规模化鸡场疑似病料151份,通过病原菌分离鉴定及生化试验,鉴定出鸡大肠埃希菌109株,平均分离率为72.19%(109/151);动物试验证实,59株分离菌有致病性,占分离菌54.13%(59/109);对致病性菌株进行血清型鉴定,确定血清型种类13种38株,占致病性菌株64.41%(38/59);优势血清型分别为O15、O18、O78、O88,占定型菌株63.16%(24/38)。

关 键 词:  大肠埃希菌  血清型

Isolation and Serotype Identification of Pathogenic E.coli in Chickens from Some Regions in Ningxia
ZHANG Yan-fei , WANG Dong , WANG Yu-jiong , HE Yu-long , WANG Jian-bo , PU Jian-ming , DING Ling-jun , HU Deng-qiang.Isolation and Serotype Identification of Pathogenic E.coli in Chickens from Some Regions in Ningxia[J].Progress In Veterinary Medicine,2012,33(5):119-121.
Authors:ZHANG Yan-fei  WANG Dong  WANG Yu-jiong  HE Yu-long  WANG Jian-bo  PU Jian-ming  DING Ling-jun  HU Deng-qiang
Institution:1.The Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in the Western China,Ningxia University,Yinchuan,Ningxia,750021,China;2.The Agriculture Bureau of Yinchuan, Yinchuan,Ningxia,750021,China;3.The Animal Husbandry Bureau of Wuzhong,Wuzhong,Ningxia,750021,China; 4.BoAo Pharmaceutical Co.Limited of Ningxia,Yinchuan,Ningxia,750021,China)
Abstract:To investigate the prevalence of the chicken colibacillosis in Ningxia,151 suspected samples were collected from 103 scaled chicken farms in 4 regions of Ningxia.Among the 151 samples,the 109 E.coli strains were isolated and identified through germiculture and biochemistry experiments,and the percentage rate of isolation was 72.19%(109/151).The results of pathogenicity experiment of animals indicated that the 59 strains were pathogenic.These strains were about 54.13%(59/109)of the total strains.Furthermore,the 38 strains of 13 serotypes,64.41% of the all infectious strains,were identified in the total 59 E.coli strains.And the most common serotypes were O15,O18,O78 and O88 at the 63.16% of the identified strains.
Keywords:chicken  E  coli  serotype
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