游泳应激对小鼠血液和肝脏脂质过氧化水平的影响

为探讨游泳应激对小鼠血液和肝脏脂质过氧化水平的影响,试验选取50只昆明小鼠进行游泳试验,于游泳前和游泳10、20、30、50min后分别随机选取10只小鼠眼眶采血致死,采集血液和肝脏,测定样品中MDA含量、SOD和GSH-Px活性。结果表明,游泳应激使小鼠血清MDA含量持续升高并显著高于游泳前对照组(P<0.05);SOD活性先显著下降(P<0.05),之后缓慢升高,游泳50min后仍显著低于对照组(P<0.05);10min游泳应激使小鼠血清GSH-Px活性显著上升(P<0.05),之后缓慢下降,50min后与对照组无显著差异。游泳应激使小鼠肝脏MDA含量显著升高(P<0.05),游泳50min后肝脏SOD活性显著升高(P<0.05),GSH-Px活性在游泳20、30、50min后均显著下降(P<0.05)。结果提示游泳应激能显著影响小鼠血液和肝脏组织脂质过氧化水平。

Effects of Swimming Stress on Lipid Peroxidation of Blood and Liver in Mice

In order to research the effects of swimming stress on lipid peroxidation of blood and liver in mice,50 Kunming mice were conducted in a swimming experiment,10 mice were randomly sacrificed before swimming and at 10 min,20 min,30 min,50 min after swimming respectively.The serum and liver tissues of mice were collected,maleic dialdehyde(MDA) contents,superoxide dismutase(SOD) activities and glutathione peroxidase(GSH-Px) activities were determined by spectrophotometry.The results showed that serum MDA contents after swimming were increased continually and significantly higher than that of before swimming(P<0.05),SOD activities were decreased markedly at 10 min after swimming(P<0.05) and then rised slightly,and SOD activities at 50 min after swimming were still significantly lower than that of before swimming(P<0.05),GSH-Px activities were increased after the swimming markedly(P<0.05),and then decreased slowly at 20 min and 30 min after swimming,there were no significant difference between the 50 min after swimming and the control group.Liver MDA content in mice increased significantly because swimming stress(P<0.05),SOD activities at 50 min after swimming rised significantly(P<0.05),GSH-Px activities decreased markedly at 20 min,30 min,50 min after swimming(P<0.05).These results indicated that swimming stress has a significant effect on lipid peroxidation of blood and liver in mice.