角膜缘干细胞缺失病理模型不同手术方法的比较

为探索一种制作角膜缘干细胞缺损病理模型的理想手术方法。采用“一刀法”、“一剪法”和“刀剪结合法”制作家兔和山羊角膜缘干细胞缺损病理模型,用植床外观特征比较手术效果。结果表明,“一刀法”完成的角巩缘切口整齐美观,但植床表面的切痕深浅不一;“一剪法”完成的角巩缘切口痕迹不显且不规整,而植床表面平整;说明“刀剪结合法”完成的角巩缘切口整齐美观且植床表面平整。说明“刀剪结合法”是制作角膜缘干细胞缺损病理模型的理想手术方法。

Effects of Limbal Stem Cells Deficiency Pathological Model by Different Surgical Methods

The objective of present paper is to explore an ideal surgical method for generation of limbal stem cells deficiency model."One scalpel method","one scissors method"and "scalpel-scissors combination method" were used to create limbal stem cells deficiency model of rabbit and goat;The features of limbal stem cell deficiency model by different surgical methods were compared.The results showed that the edge of limbo-sclera created by "one scapel medthod" was tidy,but the surface of corneal stratum was full of bumps and holes.The edge of limbo-sclera created by "one scissors method" was untidy,but the surface of corneal stratum was smooth.The edge of limbo-sclera created by "scalpel-scissors combination method" was tidy,and the surface of corneal stratum was smooth.In conclusion,"scalpel-scissors combination method" is an ideal method to create limbal stem cell deficiency model.