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牛病毒性腹泻病毒血清抗体检测
引用本文:王文,张娟,员丽娟,季新成.牛病毒性腹泻病毒血清抗体检测[J].动物医学进展,2011,32(9):51-53.
作者姓名:王文  张娟  员丽娟  季新成
作者单位:1. 新疆动物卫生监督所,新疆乌鲁木齐,830063
2. 奇台县动物疫病预防控制中心,新疆奇台,831800
3. 新疆出入境检验检疫局,新疆乌鲁木齐,830063
基金项目:新疆维吾尔自治区高技术研究发展项目(201010101);国家质量监督检验检疫总局科研项目(2011IK017)
摘    要:采用Svanova公司牛病毒性腹泻病毒血清抗体ELISA抗体检测试剂盒对采自新疆10个不同地区的875份牛血清进行了牛病毒性腹泻病毒血清抗体检测,共检出抗体阳性血清759份,最高阳性率为100%,最低为55.0%,平均为86.7%.检测结果表明该病已在新疆普遍存在,感染没有明确的地域分布规律.根据ELISA检测结果,从...

关 键 词:牛病毒性腹泻病毒  血清抗体  ELISA  病毒中和试验

Serum Antibody Detection of Bovine Viral Diarrhea Virus
WANG Wen,ZHANG Juan,YUAN Li-juan,JI Xin-cheng.Serum Antibody Detection of Bovine Viral Diarrhea Virus[J].Progress In Veterinary Medicine,2011,32(9):51-53.
Authors:WANG Wen  ZHANG Juan  YUAN Li-juan  JI Xin-cheng
Institution:1.Xinjiang Animal Health Supervise Station,Urumqi,Xinjiang,830063,China;2 Qitai County Animal Disease Prevent and Control Centre,Qitai,Xinjiang,831800;3.Xinjiang Exit-import Inspection and Quarantine Bureau,Urumq,Xinjiang,830063,China)
Abstract:In the study,875 serum samples came from 10 different areas or farms,antibody against Bovine viral diarrhea virus(BVDV) was detected used svanova company indirect ELISA kit,759 serum samples were conformed positive.The positive rate was between 55.0% and 100%,and the mean positive rate was 86.7%.The results showed that BVDV is extensive exist in Xinjiang province,and the infection rate no obvious different among different regions.15 samples were chosen and tested by virus neutralization(VN) method,the results showed that the ELISA detection was more sensitive compared with VN test.
Keywords:BVDV  serum antibody  ELISA  VN
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