| 利用菌落多重PCR对传染性胸膜肺炎放线杆菌进行血清分型 |
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| 引用本文： | 赵耘,杜昕波,李伟杰,陈敏,康凯.利用菌落多重PCR对传染性胸膜肺炎放线杆菌进行血清分型[J].动物医学进展,2010,31(1):9-12. |
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| 作者姓名： | 赵耘 杜昕波 李伟杰 陈敏 康凯 |
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| 作者单位： | 中国兽医药品监察所,北京,100081 |
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| 基金项目： | 国家科技支撑计划(2006BAD06A11) |
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| 摘 要： | 参照文献报道的传染性胸膜肺炎放线杆菌的特异基因合成5对特异引物,建立传染性胸膜肺炎放线杆菌血清型分型的菌落多重PCR方法,结果为10株传染性胸膜肺炎放线杆菌血清型参考菌株均扩增出了相应的预期片段,而支气管败血波氏杆菌、多杀性巴氏杆菌、大肠埃希菌的扩增均为阴性。利用此多重PCR方法对41株传染性胸膜肺炎放线杆菌分离菌株进行血清型分型,结果所有菌株均扩增出了相应的特异片段,其中6株为1型,5株为7型,1株为5型,29株为9型。
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| 关 键 词： | 传染性胸膜肺炎放线杆菌 菌落多重PCR 血清型 分型 |
Identification and Seroptyping of Actinobacillus pleuropneumoniae by the Colony Multiplex PCR |
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| ZHAO Yun,DU Xin-bo,LI Wei-jie,CHEN Min,KANG Kai.Identification and Seroptyping of Actinobacillus pleuropneumoniae by the Colony Multiplex PCR[J].Progress In Veterinary Medicine,2010,31(1):9-12. |
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| Authors: | ZHAO Yun DU Xin-bo LI Wei-jie CHEN Min KANG Kai |
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| Institution: | China Institute of Veterinary Drug Control;Beijing;100081;China |
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| Abstract: | Five pairs of primers against specific gene were designed and the colony multiplex PCR of identification and serogrouping of Actinobacillus pleuropneumoniae were developed.10 Actinobacillus pleuropneumoniae reference strains including serotype 1-10 were identified using the colony multiplex PCR assay.The expected sequences were obtained successfully by the colony multiplex PCR assay.But the sequences were not obtained from E.coli,B.bronchiseptica and P.multocida.41 Actinobacillus pleuropneumoniae strains we... |
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| Keywords: | Actinobacillus pleuropneumoniae colony multiplex PCR serotype serotyping |
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