| PCR技术检测猪伪狂犬病毒及其潜伏感染部位的研究 |
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| 引用本文： | 周复春.PCR技术检测猪伪狂犬病毒及其潜伏感染部位的研究[J].动物医学进展,1997,18(2):22-25. |
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| 作者姓名： | 周复春 |
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| 作者单位： | 华中农业大学畜牧兽医学院 |
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| 摘 要： | 本研究合成了伪狂犬毒糖蛋白ｇｐ５０基因引笺，该引物能够扩增糖蛋白ｇｐ５０基因中４３４－６５１之间的２１７ｂｐＤＮＡ片段，该片段含 ＳａｌＩ酶切位点，应用引物对几种不同的伪狂犬病毒株多聚酶链式反应扩增结果全为阳性。
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| 关 键 词： | PCR 检测 伪狂犬病毒 病毒 |
Studies on the Detection of Porcine Pseudorabies Virus and Its Latency Infection Sites Using PCR Technique |
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| Zou Fuchun,Chen Huanchun,Li Xuewu Fangliurong,Wu Bin,Huai Jisen.Studies on the Detection of Porcine Pseudorabies Virus and Its Latency Infection Sites Using PCR Technique[J].Progress In Veterinary Medicine,1997,18(2):22-25. |
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| Authors: | Zou Fuchun Chen Huanchun Li Xuewu Fangliurong Wu Bin Huai Jisen |
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| Abstract: | A pair of primers within pseudorabies virus glycoprotein gp50 gene was synthesized to amplify specifically a short DNA fregment (217bp) with one Sal I site. When the gnome DNA of various PRV Strains were used to amplify, all the results were positive,The 161 samples from 46 pigs, including naturally infected pigs,experimently infected pigs and stillborn fetus resulting from PRV,were used to amplify,the results of 71 samples from 46 pigs were positive,but the control samples of ORFV,IBRV,PPV ,BHK 21 Cell Were Negative. |
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| Keywords: | polymerase chain reaction detection pseudorabies virus |
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