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| 马流感病毒多重RT-PCR检测方法的建立 | |
| 引用本文： | 郭巍,戴伶俐,李雪峰,周建华,相文华,沈荣显,刘娣.马流感病毒多重RT-PCR检测方法的建立[J].动物医学进展,2008,29(11). |
| 作者姓名： | 郭巍 戴伶俐 李雪峰 周建华 相文华 沈荣显 刘娣 |
| 作者单位： | 1. 黑龙江省农业科学院东北林业大学博士后工作站,黑龙江哈尔滨,150001;中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室大动物病研究室,黑龙江哈尔滨,150001 2. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室大动物病研究室,黑龙江哈尔滨,150001 3. 黑龙江省农业科学院东北林业大学博士后工作站,黑龙江哈尔滨,150001 |
| 基金项目： | 黑龙江省博士后资助项目(LRB05-383) |
| 摘 要： | 根据马流感病毒的M基因和HA基因的保守序列,设计了两对引物,MF和MR为通用引物,可以检测出H3N8和H7N7亚型EIV,目的片段227bp,HF和HR为H3N8亚型特异性引物,目的片段595bp。利用这两对引物,通过对多重RT-PCR扩增条件的优化,建立了快速检测鉴别H3N8亚型EIV的多重RT-PCR技术。特异性和敏感性试验结果表明,该技术可以用于EIV的快速诊断和亚型鉴定。 |
| 关 键 词： | 马流感病毒 多重RT-PCR 建立 |
Establishment of Multiplex RT-PCR for the Detection of Equine influenza virus |
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| GUO Wei,DAI Ling-li,LI Xue-feng,ZHOU Jian-hua,XIANG Wen-hua,SHEN Rong-xian,LIU Di.Establishment of Multiplex RT-PCR for the Detection of Equine influenza virus[J].Progress In Veterinary Medicine,2008,29(11). | |
| Authors: | GUO Wei DAI Ling-li LI Xue-feng ZHOU Jian-hua XIANG Wen-hua SHEN Rong-xian LIU Di |
| Institution: | 1.Postdoctoral Workstation of Heilongjiang Aacdemy of Agricultural Sciences;Northeast Forestry University;Harbin;Heilongjiang;150001;China;2.Division of Livestock Infectious Diseases;National Key Laboratory of Veterinary Biotechnology;Veterinary Research Institute;CAAS;China |
| Abstract: | Two pairs of primers were designed based on the highly conserved region of M gene and HA gene of equine influenza virus(EIV).MF and MR were able to identify the H3N8 and H7N7 EIV,and the amplified segment was 227 bp.HF and HR were specific to H3N8 subtype EIV,and the amplified segment was 595 bp.The two pairs of primers were used and a multiplex RT-PCR was developed and optimized to detect EIV and identify the subtypes.The sensitivity and specificity tests were used to judge the validity of the RT-PCR. |
| Keywords: | Equine influenza virus multiplex RT-PCR establishment |
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