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猪细小病毒SC-1株VP2基因的克隆及生物信息学分析
引用本文:罗燕,郭万柱,刘艳丽,殷华平.猪细小病毒SC-1株VP2基因的克隆及生物信息学分析[J].动物医学进展,2005,26(8):87-91.
作者姓名:罗燕  郭万柱  刘艳丽  殷华平
作者单位:1. 四川农业大学动科院动物生物技术中心,四川,雅安,625014;四川农业大学都江堰分校资源环境系,四川,都江堰,611830
2. 四川农业大学动科院动物生物技术中心,四川,雅安,625014
摘    要:根据PPVNADL-2株基因组序列设计了一对引物,以复制型DNA为模板,通过PCR扩增出长约2.0kb的DNA片段,将其插入克隆载体质粒pUC19,构建重组质粒pPVP2,测序显示PPV-SC-1VP2基因全长1740bp,共编码579个氨基酸。多序列比对结果显示,猪细小病毒VP2基因保守性强,仅存在个别的差异;密码子偏向性分析结果表明,PPV-SC-1VP2基因在同一氨基酸的不同密码子的选择上存在一定的偏向性,并且与PPV-SC-1NS1在密码子的偏向性上具有相同的属性,主要偏向于使用以A结尾的密码子;氨基酸疏水性分析表明,PPV-SC-1VP2蛋白在77~82、291~304、362~373、400~411、465~477等位点存在较明显的亲水区段;跨膜区结构分析显示该蛋白不存在显著意义的跨膜区;分析该蛋白的抗原位点可能位于60~68、81~88、266~275、351~357、398~404位点处。

关 键 词:猪细小病毒  猪细小病毒SC1  VP2基因  克隆  生物信息学
文章编号:1007-5038(2005)08-0087-0S
收稿时间:2005-04-06
修稿时间:2005年4月6日

The Cloning and Bioinformation Analysis of VP2 Gene of Porcine parvovirus Strain SC-1
LUO Yan,GUO Wan-zhu,LIU Yan-li,YIN Hua-ping.The Cloning and Bioinformation Analysis of VP2 Gene of Porcine parvovirus Strain SC-1[J].Progress In Veterinary Medicine,2005,26(8):87-91.
Authors:LUO Yan  GUO Wan-zhu  LIU Yan-li  YIN Hua-ping
Abstract:According to the sequence of PPV NADL-2, a pair of primers were designed.After amplification from PPV-SC-1 RF-DNA,a 2.0 kb long fragment was insertd in pUC19 vector, named pPVP2. The sequence was determinated.The whole VP2 gene is 1740 bp long and codes 579 amino acids. Multiple sequence alignment showed that there was little difference between PPV-SC-1 vp2 and the others.Codons bias analysis showed that PPV-SC-1 VP2 biases in some codons, especially those codons whose third were A. Hydropathicity analiysis showed that there existed hydropathicity region in 77-82,291-304,362-373,400-411,465-477 sites of the PPV-SC-1 VP2.There was no transmembrane region in PPV-SC-1 VP2 by TMPRED. There maybe exist antigenicity sites in 60-68,81-88,266-275,351-357,398-404 of the PPV-SC-1 VP2.
Keywords:Porcine parvovirus  PPV-SC-1  VP2 gene  cloning  bioinformation
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