PRRSV的分离鉴定及与其他5种猪病毒混合感染情况调查

应用PCR检测方法,对来自山东、河北、河南等10省44份表现高热病症状的临床发病猪病料,进行猪繁殖与呼吸综合征病毒(PRRSV)、猪瘟病毒(CSFV)、猪圆环病毒(PCV)、猪伪狂犬病病毒(PRV)、猪巨细胞病病毒(PCMV)和猪流感病毒(SIV)6种病原的检测。结果发现,6种病原中PRRSV的检出率最高为72.7%;单纯PRRSV感染的比例仅为2.27%。根据PCR检测结果分析PRRSV与其他5种病毒的混合感染情况,结果发现两重感染中PRRSV/PRV并发率最高(62.50%),三重感染中PRRSV/PRV/PCV并发率最高(43.75%),四重感染中PRRSV/PRV/PCV/PCMV并发率最高(28.125%),五重感染中PRRSV/PCV/PCMV/CSFV/PRV并发率最高(15.625%),PRRSV/PCV/PCMV/CSFV/PRV/SIV六重感染的比例为9.375%。将PRRSV阳性病料接种猪肺巨噬细胞和Marc-145细胞,共分离到9株PRRSV,分离率为28.1%。结果表明,发病猪多为感染PRRSV的同时混合感染其他多种病毒,为制定合理的免疫程序预防猪场各种疾病提供科学依据。

Isolation and Identification of PRRSV and Investigation of Mixed Infection between PRRSV and Other Five Viruses

The PRRSV,CSFV,PCV,PRV,PCMV and SIV were detected by PCR from 44 clinical samples from ten provinces including Shandong,Hebei,and Henan etc,and the mixed infection information among PRRSV and CSFV,PCV,PRV,PCMV,SIV were analysed.The results showed that the incidence of PRRSV was highest in these six viruses and its detection rate was up to 72.7%,single infection rate of PRRSV was only 2.27%.The mixed infection information analysis among PRRSV and other viruses indicated that PRRSV/PRV was highest in the co-i...