首页 | 本学科首页   官方微博 | 高级检索  
     检索      

PRRSV ORF5基因和CSFV E2基因重组真核表达质粒的构建及小鼠免疫试验
引用本文:杨柳絮,侯磊,周宇,张森涛.PRRSV ORF5基因和CSFV E2基因重组真核表达质粒的构建及小鼠免疫试验[J].动物医学进展,2009,30(6).
作者姓名:杨柳絮  侯磊  周宇  张森涛
作者单位:1. 西北农林科技大学动物医学院,陕西杨陵,712100
2. 延安市动物疫病预防控制中心,陕西延安,716000
基金项目:陕西省自然科学基金项目 
摘    要:根据猪繁殖与呼吸综合征病毒(PRRSV)、猪瘟病毒(CSFV)已知序列设计3对引物,采用RTPCR方法扩增PRRSV ORF5基因和CSFV E2基因,目的基因依次插入真核表达载体pcDNA4.0,经PCR、酶切及测序分析,重组质粒构建成功,并命名为pcDNA4.0-ORF5-E2.重组质粒回收提纯后,以100 μg/只剂量免疫小鼠,免疫3次.三免后10 d采血并制备血清,用间接ELISA检测小鼠血清中PRRSV及CSFV抗体效价.结果显示,所构建的重组质粒能够诱导小鼠产生PRRSV及CSFV抗体.

关 键 词:猪繁殖与呼吸综合征病毒  猪瘟病毒  ORF5基因  E2基因  真核表达质粒  抗体效价

Construction of Recombinant Plasmid pcDNA4.0-ORF5-E2 of PRRSV and CSFV and the Immune Test in Mice
YANG Liu-xu,HOU Lei,ZHOU Yu,ZHANG Miao-tao.Construction of Recombinant Plasmid pcDNA4.0-ORF5-E2 of PRRSV and CSFV and the Immune Test in Mice[J].Progress In Veterinary Medicine,2009,30(6).
Authors:YANG Liu-xu  HOU Lei  ZHOU Yu  ZHANG Miao-tao
Institution:YANG Liu-xu1,HOU Lei1,ZHOU Yu2,ZHANG Miao-tao1(1.College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi,712100,China,2.Yan'an Center for Animal Disease Control and Prevention,Yan'an,716000,China)
Abstract:Based on the published nucleotide sequences of porcine reproductive and respiratory syndrome virus(PRRSV) and classical swine fever virus(CSFV),three pairs of primers were designed and used to amplify ORF5 and E2 genes by RT-PCR,and the amplified products were directionally cloned into eukaryotic expression plasmid pcDNA4.0 to construct recombinant plasmid.The recombinant plasmid pcDNA4.0-ORF5-E2 was validated by PCR,enzyme digestion and sequencing.Then,10 Kunming mice were inoculated with the purified pcDN...
Keywords:PRRSV  CSFV  ORF5 gene  E2 gene  eukaryotic expression plasmid  antibody titer  
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号