| 猪流感病毒H1N1广东分离株HA基因的克隆与进化分析 |
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| 引用本文： | 骆建才,刘义志,尚辉琴,冀君,谢青梅.猪流感病毒H1N1广东分离株HA基因的克隆与进化分析[J].动物医学进展,2010,31(10). |
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| 作者姓名： | 骆建才 刘义志 尚辉琴 冀君 谢青梅 |
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| 作者单位： | 1. 深圳市农牧实业有限公司,广东深圳,518023
2. 华南农业大学动物科学学院,广东广州,510642 |
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| 基金项目： | 广东省重大科技专项项目 |
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| 摘 要： | 采用常规的血清学试验和特异性RT-PCR,从广东不同地区猪场分离鉴定出8株H1N1亚型猪流感病毒(SIV)。用流感病毒血凝素(HA)基因通用引物扩增了8株病毒的血凝素(HA)基因,经克隆测序,HA基因全长1 757 bp,编码566个氨基酸。8个毒株的HA基因推导氨基酸序列分析表明,均含有8个潜在的N糖基化位点,且糖基化位点相同,其HA1、HA2之间切割位点序列为IPSIQSR↓G,从分子水平推论,此8株H1N1 SIV均属于非高致病性毒株。同源性分析表明,此8株病毒的氨基酸序列与经典SIV之间的同源性在92.3%~94.7%之间;与2009年甲型H1N1流感病毒同源性在80.4%~92.4%之间;与欧洲类禽SIV分离株同源性在80.4%~84.1%之间。进化关系表明,该8株SIV与A-swine-Shanghai-3-2005-H1N1同处一分支,与2009年甲型H1N1流感病毒和经典SIV分离株亲缘关系较近,与欧洲类禽SIV分离株亲缘关系较远。
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| 关 键 词： | 甲型H1N1流感病毒 猪流感病毒 HA基因 进化分析 |
Cloning and Phylogenetic Analysis of HA Gene of H1N1 Swine Influenza Virus Strains from Guangdong |
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| LUO Jian-cai,LIU Yi-zhi,SHANG Hui-qin,JI Jun,XIE Qing-mei.Cloning and Phylogenetic Analysis of HA Gene of H1N1 Swine Influenza Virus Strains from Guangdong[J].Progress In Veterinary Medicine,2010,31(10). |
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| Authors: | LUO Jian-cai LIU Yi-zhi SHANG Hui-qin JI Jun XIE Qing-mei |
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| Institution: | LUO Jian-cai1,LIU Yi-zhi2,SHANG Hui-qin2,JI Jun2,XIE Qing-mei2 (1.Agriculture and Animal Husbandry Industry Co.,Ltd.of Shenzhen City,Shenzhen,Guangdong,518023,China,2.College of Animal Science,South China Agricultural University,Guangzhou,510642,China) |
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| Abstract: | Using the serologic assay and the method of specific RT-PCR,8 strains of H1N1 subtype swine influenza viruses were isolated from different area of Guangdong province in 2009.8 strains of the virus hemagglutinin(HA) genes were amplified by universal primer.The whole length of HA genes of all 15 isolates were composed of 1 757 bp,coding 566 amino acids.The deduced amino acid sequence of HA gene had eight potential N-glycosylation sites and the glycosylation sites are the same.It was found that the cleavage si... |
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| Keywords: | H1N1 subtype Swine influenza virus HA gene phylogenetic analysis |
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