| 番鸭源鹅细小病毒ZQ株VP2基因的克隆和序列分析 |
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| 引用本文： | 宋永峰,周丽,周全,高恒,宋延华,温纳相.番鸭源鹅细小病毒ZQ株VP2基因的克隆和序列分析[J].动物医学进展,2009,30(7):42-45. |
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| 作者姓名： | 宋永峰 周丽 周全 高恒 宋延华 温纳相 |
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| 作者单位： | 广东温氏食品集团研究院,广东新兴,527400 |
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| 摘 要： | 根据国内外已发表的鹅细小病毒(GPV)B株和GD株基因序列,应用DNA Star分子生物学软件设计一对引物,应用PCR技术扩增GPV ZQ株的结构蛋白基因VP2全基因片段。将扩增得到的VP2全基因克隆到pMD18-T载体上,获得的重组质粒经PCR鉴定后进行序列测定。结果表明ZQ株基因大小为1 764 bp,编码587个氨基酸,其基因序列与GPV参考毒株推导的氨基酸序列同源性在89.1%~99.3%之间,差异较大;与番鸭细小病毒(MDPV)参考毒株相比同源性在92.0%~92.5%之间,超过与部分GPV毒株的同源性,推测可能与该毒株来源于番鸭而不是鹅有关,另外也在基因水平上解释了GPV与MDPV在血清学上存在交叉反应的部分原因。
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| 关 键 词： | 鹅细小病毒 VP2基因 克隆 序列分析 |
Cloning and Sequence Analysis of VP2 Gene of Goose Parvovirus ZQ Strain Isolated from Muscovy Duck |
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| SONG Yong-feng,ZHOU Li,ZHOU Quan,GAO Heng,SONG Yan-hua,WEN Na-xiang.Cloning and Sequence Analysis of VP2 Gene of Goose Parvovirus ZQ Strain Isolated from Muscovy Duck[J].Progress In Veterinary Medicine,2009,30(7):42-45. |
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| Authors: | SONG Yong-feng ZHOU Li ZHOU Quan GAO Heng SONG Yan-hua WEN Na-xiang |
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| Institution: | Guangdong Wen's Foodstuffs Group Company Research Institute;Xinxing;Guangdong;527400;China |
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| Abstract: | A pair of primer were designed by DNAstar software to amplify VP2 gene of goose parvovirus(GPV)ZQ strain by PCR according to the published sequence of GPV B strain and GD strain in GenBank.VP2 gene amplified by PCR was cloned into the pMD18-T vector,then sequenced and analyzed after identification by PCR of recombinant plasmid.The result of sequence showed that VP2 gene included 1 764 bp,which encoded 587 amino acids.The gene shared 89.1%-99.3% homology to GPV strains and 92.0%-92.5% homology to Muscovy duc... |
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| Keywords: | Goose parvovirus VP2 gene cloning sequence analysis |
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