| 1株牛传染性鼻气管炎病毒的分离鉴定 |
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| 引用本文： | 唐泰山,王凯民,邓碧华,张常印,雷治海.1株牛传染性鼻气管炎病毒的分离鉴定[J].动物医学进展,2006,27(6):69-71. |
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| 作者姓名： | 唐泰山 王凯民 邓碧华 张常印 雷治海 |
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| 作者单位： | 1. 江苏出入境检验检疫局动物检疫实验室,江苏南京210001
2. 南京农业大学动物医学院,江苏南京210095 |
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| 基金项目： | 江苏省检验检疫局科技基金 |
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| 摘 要： | 在对进口种用奶牛隔离检疫期间,从1头IBRV中和抗体阳性奶牛中分离出1株病毒。该分离株表现类似于IBRV特征的细胞病变,细胞圆缩,聚集成葡萄串样群落,在单层细胞上形成空洞。用特异性抗IBRV阳性血清与其进行中和试验,发现IBRV标准阳性血清对分离株的中和抗体滴度为27,与IBRV标准株的中和抗体滴度相差不到1个滴度。用OIEV推荐的IBR特异性引物对分离病毒进行PCR扩增,获得与设计基因片段大小一致的特异性条带,表明分离到的病毒为IBRV。
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| 关 键 词： | 牛传染性鼻气管炎病毒 分离鉴定 中和试验 聚合酶链反应 |
| 文章编号： | 1007-5038（2006）06-0069-03 |
| 收稿时间： | 2005-09-21 |
| 修稿时间： | 2005年9月21日 |
Isolation and Identification of One Strain of Infectious bovine rhinotracheitis virus |
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| TANG Tai-shan,WANG Kai-min,Deng Bi-hua,ZHANG Chang-yin,LEI Zhi-hai.Isolation and Identification of One Strain of Infectious bovine rhinotracheitis virus[J].Progress In Veterinary Medicine,2006,27(6):69-71. |
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| Authors: | TANG Tai-shan WANG Kai-min Deng Bi-hua ZHANG Chang-yin LEI Zhi-hai |
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| Institution: | 1. Lab of Animal Quarantine, APHIC, Jiangsu Entry-Exit Inspection And Quarantine Bureau ,Nanjing , Jiangsu, 210001 ,China ; 2. College of Veterinary Medicine, Nanjing Agriculture University, Nanjing , Jiangsu, 210095, China |
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| Abstract: | During the quarantine of imported cows,one strain of virus was isolated from a cow which Infectious bovine rhinotracheitis virus neutralization antibody was positive.The virus reproduced in MDBK cell and induced cytopathogenic effect(CPE) coincident with IBRV's.The neutralizing antibody titer was 2~7 after virus neutralization test was performed by the IBRV specificity serum.The PCR amplification yielded a special band for the virus when using the pair of primers according to the sequence of IBRV.The result showed that the virus was IBRV. |
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| Keywords: | Infectious bovine rhinotracheitis virus isolation and identifization neutralization test PCR |
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