| 李斯特菌套式PCR快速检测方法的建立及初步应用 |
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| 引用本文： | 马保华,张辉华,吕平,曹永长,毕英佐.李斯特菌套式PCR快速检测方法的建立及初步应用[J].动物医学进展,2005,26(11):57-60. |
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| 作者姓名： | 马保华 张辉华 吕平 曹永长 毕英佐 |
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| 作者单位： | 南海出入境检验检疫局,广东佛山,528200;华南农业大学动物科学学院,广东广州,510642;佛山科技学院,广东佛山,528000;华南农业大学动物科学学院,广东广州,510642 |
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| 基金项目： | 国家质量监督检验检疫总局资助项目 |
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| 摘 要： | 根据GenBank数据库的单核细胞增生李斯特菌iap基因设计2对引物,建立了套式PCR快速检测单增李斯特菌的方法。两对引物分别扩增出约1 500 bp和500 bp片段,与预期大小一致。套式PCR方法灵敏性实验检测极限为10 cfu/mL;人工污染猪肉检测极限为103cfu/mL;整个检测过程可在7 h内完成。采用该法对南海口岸进口的冻肉进行检测,检测出9份阳性,与传统的细菌分离方法完全一致,说明套式PCR方法具有很好的特异性。
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| 关 键 词： | 单核细胞增生李斯特菌 套式PCR 快速检测 |
| 文章编号： | 1007-5038（2005）11-0057-04 |
| 收稿时间： | 2005-06-14 |
| 修稿时间： | 2005年6月14日 |
Detection of L.monocytogens by Nested-PCR Assay and Its Preliminary Application |
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| MA Bao-hua,ZHANG Hui-hua,L Ping,CAO Yong-chang,BI Ying-zuo.Detection of L.monocytogens by Nested-PCR Assay and Its Preliminary Application[J].Progress In Veterinary Medicine,2005,26(11):57-60. |
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| Authors: | MA Bao-hua ZHANG Hui-hua L Ping CAO Yong-chang BI Ying-zuo |
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| Institution: | MA Bao-hua,ZHANG Hui-hua,L(U) Ping,CAO Yong-chang,BI Ying-zuo |
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| Abstract: | According to the sequence data published by Genbank,two pairs of primers were designed to amplify the iap gene of Listeria monocytogens and a Nested polymerase chain reaction(Nested-PCR) method for the detection of Listeria monocytogens has been developed.Two pairs of primers amplified 1 500 bp and 500 bp fragment respectively as expected.The detection limit is 10~1 cfu/mL,while the detection limit is 10~3 cfu/mL when detecting the artificially contaminated swine meat.The whole detection can be finished in 7 hours.9 listeria monocytogens were detected from the import frozen meat,which is accorded with that of the ISO11290-1 method. |
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| Keywords: | nested-PCR L monocytogens rapid detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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