| 鸡Mx基因的原核表达及表达蛋白抗病毒活性初步研究 |
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| 引用本文： | 陈胜锋,王丙云,高健潜,陈志胜,计慧琴,陈金顶.鸡Mx基因的原核表达及表达蛋白抗病毒活性初步研究[J].动物医学进展,2011,32(4):6-9. |
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| 作者姓名： | 陈胜锋 王丙云 高健潜 陈志胜 计慧琴 陈金顶 |
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| 作者单位： | 佛山科学技术学院动物医学系;华南农业大学兽医学院; |
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| 基金项目： | 佛山市科技发展专项基金(31-2-1); 佛山市产学研基金(2006A031) |
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| 摘 要： | 将鸡Mx基因克隆入pET一32a载体,筛选鉴定后将重组阳性质粒转化到大肠埃希菌株BL21进行诱导表达,表达产物用west,ern blot鉴定,并经镍亲和层析法纯化及透析复性,得到的蛋白用微量细胞病变抑制法检测其抗病毒活性.结果显示,构建的载体经PCR扩增得到长度为2 118 bp的特异片段,测序结果证实与鸡Mx基因同...
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| 关 键 词： | Mx蛋白 原核表达 抗病毒活性 鸡 |
Prokaryotic Expression of Chick Mx Gene and Its Antiviral Activity |
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| CHEN Sheng-feng,WANG Bin-yun,GAO Jian-qian,CHEN Zhi-sheng,JI Hui-qin,CHEN Jin-ding.Prokaryotic Expression of Chick Mx Gene and Its Antiviral Activity[J].Progress In Veterinary Medicine,2011,32(4):6-9. |
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| Authors: | CHEN Sheng-feng WANG Bin-yun GAO Jian-qian CHEN Zhi-sheng JI Hui-qin CHEN Jin-ding |
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| Institution: | CHEN Sheng-feng1,WANG Bin-yun1,GAO Jian-qian1,CHEN Zhi-sheng1,JI Hui-qin1,CHEN Jin-ding2(1.Department of veterinary,FoShan University,Foshan,Guangdong,528231,China,2.College of Veterinary Medicine,South China Agricultural University,Guangzhou,510642,China) |
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| Abstract: | To investigate the antiviral activity of chick Mx protein,Mx gene was cloned into the prokaryotic expression vector pET-32a.The positive recombinant plasmid was transformed into E.coliBL21 strain,and then induced by IPTG.The product was identified by Western blot and purified via metal chelate affinity chromatography.The anti-viral activity of renaturated protein was detected by CPEI50.Results showed that a 2118bp PCR product,99.95% homology with chicken Mx gene,was obtained from pET-cMx.Western blot showed... |
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| Keywords: | Mx protein prokayrotic expression antiviral activity chick |
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