| 鸭源新城疫病毒的分离鉴定 |
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| 引用本文： | 宋战胜,王晶钰,赵伟,李亮,邓小敏.鸭源新城疫病毒的分离鉴定[J].动物医学进展,2007,28(5):22-25. |
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| 作者姓名： | 宋战胜 王晶钰 赵伟 李亮 邓小敏 |
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| 作者单位： | 西北农林科技大学动物科技学院,陕西杨陵,712100 |
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| 摘 要： | 从病死肉鸭肝脏中分离到2株病毒(ZH1、ZH2),均能够凝集鸡、肉鸭、绵羊、山羊、猪、人、兔、牛等的红细胞,且这种血凝性可被NDV标准阳性血清所抑制.参照NDV毒力判定标准及其方法对分离毒株ZH1、ZH2进行了鸡胚最小致死量平均死亡时间(MDT)、鸡胚半数感染量(EID50)以及1日龄鸡脑内接种致病指数(ICPI)测定,结果ZH1、ZH2株的MDT为52 h和44 h,EID50为106.4/0.1mL和108.64/0.1mL,ICPI为1.93和1.975.表明这2株分离病毒均为NDV强毒株.
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| 关 键 词： | 新城疫病毒 分离鉴定 鸭 |
| 文章编号： | 1007-5038（2007）05-0022-04 |
| 收稿时间： | 2007-03-14 |
| 修稿时间： | 2007年3月14日 |
Isolation and Identification of NDV from Duck |
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| SONG Zhan-sheng,WANG Jing-yu,ZHAO Wei,LI Liang,DENG Xiao-min.Isolation and Identification of NDV from Duck[J].Progress In Veterinary Medicine,2007,28(5):22-25. |
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| Authors: | SONG Zhan-sheng WANG Jing-yu ZHAO Wei LI Liang DENG Xiao-min |
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| Abstract: | Two strains of viruses were isolated from the infected duck's livers using chicken embryos.The two strains of viruses could agglutinate the chicken,duck,sheep,goat,swine,human,rabbit and cattle erythrocytes.The hemagglutination of viruses could be inhibited by Newcastle disease virus(NDV) antiserum.The death time of chicken embryos(MDT) of the isolated strain of ZH1 was 52 h,the intraeerebral pathogenicity index(ICPI)of it in 1-day-old chicks was 1.93,the EID50 was 106.4/0.1 mL.The MDT,ICPI and EID50 of the isolated strain of ZH2 were 44 h,1.97 and 108.64/0.1 mL,respectively.The results indicated that the two viral isolates were virulent Newcastle disease virus(NDV). |
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| Keywords: | NDV isolation and identification duck |
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