| 牛腺病毒7型PCR检测方法的建立 |
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| 引用本文： | 王进轶,刘双翼,刘合义,王红,侯喜林.牛腺病毒7型PCR检测方法的建立[J].动物医学进展,2009,30(1). |
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| 作者姓名： | 王进轶 刘双翼 刘合义 王红 侯喜林 |
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| 作者单位： | 黑龙江八一农垦大学动物科技学院,黑龙江,大庆,163319 |
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| 摘 要： | 根据GenBank中牛腺病毒7型(BAdV-7)的蛋白酶基因序列,设计一对特异性的引物,扩增该序列中775 bp的片段,建立了BAdV-7的PCR检测方法.以Fukuroi株BAdV-7 DNA为模板进行特异性和敏感性试验,结果显示,该方法检测BAdV-7 DNA最小检测量为1ng;与牛传染性鼻气管炎病毒(IBRV)、牛冠状病毒(BCV)、牛轮状病毒(BRV)、牛呼吸道舍胞体病毒(BRSV)和鸡产蛋下降综合征病毒(EDSV)无交叉反应.方法为BAdV-7感染的临床诊断和流行病学调查奠定了基础.
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| 关 键 词： | 牛腺病毒7型 诊断方法 聚合酶链反应 |
Establishment of PCR for Detection of Bovine adenovirus Type 7 |
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| WANG Jin-yi,LIU Shuang-yi,LIU He-yi,WANG Hong,HOU Xi-lin.Establishment of PCR for Detection of Bovine adenovirus Type 7[J].Progress In Veterinary Medicine,2009,30(1). |
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| Authors: | WANG Jin-yi LIU Shuang-yi LIU He-yi WANG Hong HOU Xi-lin |
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| Institution: | College of Animal Science and Technology;Heilongjiang August First Land Reclamation University;Daqing;Heilongjiang;163319;China |
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| Abstract: | One pairs of primers P1/P2 were designed using primer 5.0 program based on the published sequences of proteinase gene of BAdV-7.The specific amplication fragment is 775 bp,a PCR method for diagnosis of bovine adenovirus serotype 7 was established.This method was able to detect 1ng virus DNA and no cross reaction with BCV,IBRV,BRV,BRSV and EDSV.The technique has been applied in the clinical diagnosis and epidemiological research. |
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