| PCR对猪全血、血清、尿液和粪便中PCV2的检测 |
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| 引用本文： | 梁红虎,罗满林,刘镇明.PCR对猪全血、血清、尿液和粪便中PCV2的检测[J].动物医学进展,2004,25(1):74-76. |
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| 作者姓名： | 梁红虎 罗满林 刘镇明 |
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| 作者单位： | 华南农业大学兽医学院,广东,广州,510642;华南农业大学兽医学院,广东,广州,510642;华南农业大学兽医学院,广东,广州,510642 |
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| 摘 要： | 根据已发表的基因序列 ,选取 PCV2保守的基因片段 ,利用计算机软件设计的一对引物对某猪场的 7只患病仔猪的全血、血清、尿液、和粪便进行 PCR扩增 ,再对扩增产物酶切鉴定 ,并将 PCR产物连接到 p MD1 8-T载体上 ,然后转化到大肠杆菌 DH5α感受态细胞中 ,抽提重组质粒 ,对重组质粒 PCVp MD1 8进行 PCR、酶切鉴定及测序。结果表明 :全血、血清、尿、粪便阳性率分别为 85.7% (6/7)、71 .4% (5/7)、42 .9%(3 /7)、2 8.6% (2 /7)。通过比较 ,全血的阳性率最高 ,其次为血清
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| 关 键 词： | PCR 全血 血清 尿液和粪便 PCV2 |
| 文章编号： | 1007-5038(2004)01-0074-03 |
| 修稿时间： | 2003年6月11日 |
Testing of PCV2 in whole Blood,serum,urine,feces by PCR |
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| LIANG Hong hu,LUO Man lin,LIU Zhen ming.Testing of PCV2 in whole Blood,serum,urine,feces by PCR[J].Progress In Veterinary Medicine,2004,25(1):74-76. |
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| Authors: | LIANG Hong hu LUO Man lin LIU Zhen ming |
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| Abstract: | According to the published gene sequence of PCV2,and taking the conserved segment,a pair of primers were designed through Oligo5.0 software.The sequence was amplified from whole blood,serum,urine,feces of sick piglets obtained from a pig farmer using PCR.The PCR products were cloned into pMD18 T easy vector,then the PCVpMD18 was transformed into DH5.The recombinant plasmid PCV pMD18 was identified by PCR,restriction enzyme analysis and sequencing. It was found that the positive ratio of whole blood, serum, urine, feces is 85.7%(6/7),71.4(5/7),42.9%(3/7),28.6(2/7) respectively. The positive ratio of whole blood is highest and the positive ratio of serum is better than that of urine and feces.The positive ratio of feces is lowest. |
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| Keywords: | PCR Porcine circovirus2 whole blood serum urine feces |
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