| 猪初乳中IgA的提纯与鉴定 |
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| 引用本文： | 高进东,胡长敏,丁明星,陈建国.猪初乳中IgA的提纯与鉴定[J].动物医学进展,2006,27(5):81-83. |
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| 作者姓名： | 高进东 胡长敏 丁明星 陈建国 |
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| 作者单位： | 华中农业大学动物医学院,湖北武汉,430070 |
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| 摘 要： | 文章从猪初乳中提纯得到IgA。猪初乳经过饱和硫酸铵盐析后,依次应用Seph-adex G-200凝胶过滤、DEAE-纤维素-52离子交换和Sephadex G-200凝胶过滤层析,分离得到猪IgA,经双相扩散免疫,免疫电泳和SDS-PAGE电泳鉴定可达电泳纯。
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| 关 键 词： | 猪初乳 分离 鉴定 |
| 文章编号： | 1007-5038（2006）05-0081-03 |
| 收稿时间： | 2006-01-13 |
| 修稿时间： | 2006年1月13日 |
Preparation and Idetification of IgA from Procine Colostrum |
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| GAO Jin-dong,HU Chang-min,DING Ming-xing,CHEN Jian-guo.Preparation and Idetification of IgA from Procine Colostrum[J].Progress In Veterinary Medicine,2006,27(5):81-83. |
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| Authors: | GAO Jin-dong HU Chang-min DING Ming-xing CHEN Jian-guo |
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| Institution: | College of Animal Medicine, Huazhong Agriculture University, Wuhan ,Hubei,430070, China |
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| Abstract: | Porcine immunoglobulinA(IgA) was isolated from porcine colostrums.IgA was purified by precipition with different-saturation of ammonium sulfate(SAS),gel filtration on Sephadex G-200,chromatography on DEAE-cellulose-52 ion-exchange and gel filtration on Sephadex G-200 in order,and identified with double agar immunodiffudion,immunoelectrophoresis and SDS-PAGE.The result showed that obtained IgA was high-purificated. |
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| Keywords: | IgA |
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